Homogenization of Tissue Culture Cells in Suspension

Abstract

For this procedure, the cells are first swollen in a buffered hypo‐osmotic medium containing potassium chloride, magnesium acetate and dithiothreitol to render the plasma membrane more susceptible to subsequent homogenization. After pelleting the cells, most of the ‘swelling’ medium is removed; the cells are resuspended in the residual medium and homogenized by Dounce homogenization.

Keywords: homogenization; tissue culture; mammalial cells; monolayer cells; suspension cells

References

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Graham JM (1997) Homogenization of tissues and cells. In: Graham JM and Rickwood D (eds) Subcellular Fractionation – a Practical Approach, pp. 1–29. Oxford: Oxford University Press

Marsh M, Schmid S, Kern H et al. (1987) Rapid analytical and preparative isolation of functional endosomes by free flow electrophoresis. Journal of Cell Biology 104: 875–886.

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Graham, John M(Mar 2004) Homogenization of Tissue Culture Cells in Suspension. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003803]