Preparation of Nuclei from Rat Liver using Sucrose

Abstract

Nuclei can be prepared from rat liver using sucrose.

Keywords: liver; nuclei; sucrose separation; isopycnic centrifugation

References

Blobel G and Potter VR (1966) Nuclei from rat liver: an isolation method that combines purity with high yield. Science 154: 1662–1665.

Gollin SM and Wray W (1984) Isopycnic centrifugation of metaphase chromosomes in Nycodenz. Experimental Cell Research 152: 204–211.

Hoffmann P and Chalkley R (1978) Procedures for minimizing protease activity during isolation of nuclei, chromatin and the histones. In: Stein G, Stein J and Kleinsmith LJ (eds) Methods in Cell Biology, vol. XVII, pp. 1–12. London: Academic Press.

Rickwood D, Messent A and Patel D (1997) Isolation and characterization of nuclei and nuclear subfractions. In: Graham JM and Rickwood D (eds) Subcellular Fractionation – a Practical Approach, pp. 71–105. Oxford: IRL Press at Oxford University Press.

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How to Cite close
Graham, John M(Mar 2004) Preparation of Nuclei from Rat Liver using Sucrose. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003804]