Preparation of Nuclei from a Homogenate using a Sucrose Barrier

Abstract

Nuclei can be prepared from a homogenate using a sucrose barrier.

Keywords: nuclei; isopycnic centrifugation; sucrose; cell homogenate

References

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Gollin SM and Wray W (1984) Isopycnic centrifugation of metaphase chromosomes in Nycodenz. Experimental Cell Research 152: 204–211.

Hofmann P and Chalkley R (1978) Procedures for minimizing protease activity during isolation of nuclei, chromatin and histones. In: Stein G, Stein J and Kleinsmith LJ (eds) Methods in Cell Biology, XVII, pp. 1–12. London: Academic Press.

Rickwood D, Messent A and Patel D (1997) Isolation and characterization of nuclei and nuclear subfractions. In: Graham JM and Rickwood D (eds) Subcellular Fractionation – a Practical Approach, pp. 71–105. Oxford: IRL Press at Oxford University Press.

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How to Cite close
Graham, John M(Mar 2004) Preparation of Nuclei from a Homogenate using a Sucrose Barrier. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003805]