Preparation of Nuclei from a Homogenate using a Sucrose Barrier

John M Graham, Wirral, UK

Published online: March 2004

DOI: 10.1038/npg.els.0003805

Nuclei can be prepared from a homogenate using a sucrose barrier.

Keywords: nuclei; isopycnic centrifugation; sucrose; cell homogenate

 References
    Blobel G and Potter VR (1966) Nuclei from rat liver: an isolation method that combines purity with high yield. Science 154: 1662–1665.
    Gollin SM and Wray W (1984) Isopycnic centrifugation of metaphase chromosomes in Nycodenz. Experimental Cell Research 152: 204–211.
    book Hofmann P and Chalkley R (1978) "Procedures for minimizing protease activity during isolation of nuclei, chromatin and histones". In: Stein G, Stein J and Kleinsmith LJ (eds) Methods in Cell Biology, XVII, pp. 1–12. London: Academic Press.
    book Rickwood D, Messent A and Patel D (1997) "Isolation and characterization of nuclei and nuclear subfractions". In: Graham JM and Rickwood D (eds) Subcellular Fractionation – a Practical Approach, pp. 71–105. Oxford: IRL Press at Oxford University Press.

Related Sub-Topics:

Cell Compartments and Cellular Organelles | Techniques in Cell Biology
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Article Title: Preparation of Nuclei from a Homogenate using a Sucrose Barrier
 
How to Cite close
Graham, John M(Mar 2004) Preparation of Nuclei from a Homogenate using a Sucrose Barrier. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003805]