Isolation of a Light Mitochondrial Fraction from a Homogenate

Abstract

A light mitochondrial fraction is one of the four principal fractions obtained by differential centrifugation of a mammalian tissue or cell homogenate. It is obtained by centrifugation of a 3000g supernatant at approx. 15 000g for 15 min. It contains principally the lighter mitochondria and most of the lysosomes and peroxisomes and any intact Golgi membranes and is used as a primary step in the purification of lysosomes and peroxisomes.

Keywords: mitochondria; lysosomes; peroxisomes; liver; organelles

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Graham, John M(Mar 2004) Isolation of a Light Mitochondrial Fraction from a Homogenate. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003806]