Abstract
Mitochondria can be purified from a light mitochondrial fraction in a continuous sucrose gradient using a swinging‐bucket rotor.
Keywords: mitochondria; isopycnic centrifugation; sucrose gradients; rat liver
Isolation of Mitochondria from a Homogenate using a Sucrose Density Gradient
John M Graham, Wirral, UK
Published online: March 2004
DOI: 10.1038/npg.els.0003807
References
Arai K, Kanaseki T and Ohkuma S (1991) Isolation of highly purified lysosomes from rat liver: identification of electron carriercomponents on lysosomal membranes. Journal of Biochemistry 110: 541–547.
Beauvoit B, Rigoulet M, Guerin B and Canioni P (1989) Polyphosphates, a source of high energy phosphate in yeast mitochondria: a 31P NMR study. FEBS Letters 252: 17–21.
Diczfalusy U and Alexson SEH (1988) Peroxisomal chain shortening of prostaglandin F2. Journal of Lipid Research 29: 1629–1636.
Leighton F, Poole B, Beaufay H et al. (1968) The large scale separation of peroxisomes, mitochondria and lysosomes from the livers of rats injected with Triton WR 1339. Journal of Cell Biology 37: 482–513.
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How to Cite
Graham, John M(Mar 2004) Isolation of Mitochondria from a Homogenate using a Sucrose Density Gradient. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003807]
