Isolation of Mitochondria from a Homogenate using a Sucrose Density Gradient

John M Graham, Wirral, UK

Published online: March 2004

DOI: 10.1038/npg.els.0003807

Mitochondria can be purified from a light mitochondrial fraction in a continuous sucrose gradient using a swinging-bucket rotor.

Keywords: mitochondria; isopycnic centrifugation; sucrose gradients; rat liver

 References
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    Leighton F, Poole B, Beaufay H et al. (1968) The large scale separation of peroxisomes, mitochondria and lysosomes from the livers of rats injected with Triton WR 1339. Journal of Cell Biology 37: 482–513.

Related Sub-Topics:

Cell Compartments and Cellular Organelles | Techniques in Cell Biology
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Article Title: Isolation of Mitochondria from a Homogenate using a Sucrose Density Gradient
 
How to Cite close
Graham, John M(Mar 2004) Isolation of Mitochondria from a Homogenate using a Sucrose Density Gradient. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003807]