Isolation of Mitochondria from a Homogenate using a Sucrose Density Gradient

Abstract

Mitochondria can be purified from a light mitochondrial fraction in a continuous sucrose gradient using a swinging‐bucket rotor.

Keywords: mitochondria; isopycnic centrifugation; sucrose gradients; rat liver

References

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Leighton F, Poole B, Beaufay H et al. (1968) The large scale separation of peroxisomes, mitochondria and lysosomes from the livers of rats injected with Triton WR 1339. Journal of Cell Biology 37: 482–513.

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How to Cite close
Graham, John M(Mar 2004) Isolation of Mitochondria from a Homogenate using a Sucrose Density Gradient. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003807]