Fluorescent Labelling of Antibodies

Abstract

One of the most common fluorochromes used in immunocytochemistry and flow cytometry is fluorescein isothiocyanate (FITC), which produces a fluorescent apple‐green colour on exposure to ultraviolet light. Antibodies can be labelled with FITC via lysine residues.

Keywords: immunofluorescence; immunocytochemistry; flow cytometry; fluorescein isothiocyanate; FITC

Figure 1.

Section of umbilical vein (endothelial cells) stained with an FITC‐conjugated antibody to von Willebrand factor.

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References

Goding JW (1976) Conjugation of antibodies with fluorochromes: modifications to the standard methods. Journal of Immunological Methods 13: 215–226.

The TH and Feltkamp TEW (1970a) Conjugation of fluoroscein isothiocyanate to antibodies I. Experiments on the conditions of conjugation. Immunology 18: 865–873.

The TH and Feltkamp TEW (1970b) Conjugation of fluoroscein isothiocyanate to antibodies II A reproducible method. Immunology 18: 875–881.

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How to Cite close
Liddell, JE(Jul 2003) Fluorescent Labelling of Antibodies. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003808]