Gene Localization by in situ Hybridization: FISH

Abstract

The fluorescent detection of nucleic acid sequences ranging from single genes to the whole genome in chromosomes and interphase nuclei fixed on glass slides.

Keywords: fluorescence in situ hybridization; DNA; chromosomes; interphase nuclei

Figure 1.

A biotinylated satellite (tandem‐repeat) probe specific for the heterochromatic region of human chromosome 9 hybridized to normal human lymphocyte metaphase spread. This individual has a much larger block of heterochromatin on one chromosome 9 homologue than on the other. Detection is by avidin–FITC with one round of amplification.

Figure 2.

A biotinylated probe prepared by a human‐specific polymerase chain reaction (inter‐Alu‐PCR) from a human–rodent hybrid. The probe has been hybridized to a human metaphase spread prepared from an individual with a translocation between chromosome 9 and chromosome 22. This is an example of ‘reverse chromosome painting’ and shows that the original hybrid contained the long arm of chromosome 9 as its only human material. Detection as in Figure .

Figure 3.

This is a biotinylated cosmid probe hybridized to a metaphase spread from the same cell line used in Figure . Hybridization is to the normal 9 and to the abnormal chromosome 22, showing that the cosmid derives from chromosome 9 distal to the position of the breakpoint in the translocation. Detection as in Figure .

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References

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How to Cite close
Ekong, R, and Povey, S(Feb 2003) Gene Localization by in situ Hybridization: FISH. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003810]