Electrophoresis of DNA and RNA in Non‐denaturing Vertical Polyacrylamide Gels

Edwin M Southern, University of Oxford, Oxford, UK

Published online: May 2003

DOI: 10.1038/npg.els.0003811

Electrophoresis in non-denaturing polyacrylamide gels is a simple and useful technique for analysing RNA and DNA of less than 1000 bp. Separations are generally not affected by hidden nicks in the molecules but are subject to conformational factors of individual molecules

Keywords: denaturing; polyacrylamide gels; electrophoresis

Figure 1. Slab electrophoretic apparatus.
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 References
    Fangman WL (1978) Separation of very large DNA molecules by gel electrophoresis. Nucleic Acids Research 5: 653.
    book Jovin TM (1971) "Electrophoretic fractionation of oligodeoxyribonucleotides". In: Grossman L and Moldave K (eds) Methods in Enzymology, 21, p. 179. London and New York: Academic Press.

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
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Article Title: Electrophoresis of DNA and RNA in Non‐denaturing Vertical Polyacrylamide Gels
 
How to Cite close
Southern, Edwin M(May 2003) Electrophoresis of DNA and RNA in Non‐denaturing Vertical Polyacrylamide Gels. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003811]