Western Blotting Analysis of Translation Products

D Whitehouse, University of Hertfordshire, Hatfield, UK
J Lovegrove, University of Hertfordshire, Hatfield, UK

Published online: May 2005

DOI: 10.1038/npg.els.0003812

Western blotting (immunoblotting) is a system for the immunological detection of specific proteins that have been transferred from a polyacrylamide gel to an immobilizing membrane. Proteins transferred to immobilizing filter membranes, such as nitrocellulose, are well suited to high-sensitivity solid-phase immunoassay detection techniques.

Keywords: immunoblotting; electroblotting; immunodetection; electrophoresis

Figure 1. Analysis of phosphoglucomutase (PGM)/-galactosidase fusion protein, expressed in Escherichia coli, after separation on an SDS gel. (a) Coomassie blue-stained molecular weight markers. (b) Coomassie blue-stained E. coli protein extract. (c) Immunostained western blot showing the PGM/-gal fusion protein in the same protein extract detected using sheep anti-rabbit PGM primary antibodies.
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 References
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Related Sub-Topics:

Diagnostics, Therapeutics and Methods | Antibodies | Techniques in Cell Biology
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Article Title: Western Blotting Analysis of Translation Products
 
How to Cite close
Whitehouse, D, and Lovegrove, J(May 2005) Western Blotting Analysis of Translation Products. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003812]