Western Blotting Analysis of Translation Products
D Whitehouse, University of Hertfordshire, Hatfield, UK
J Lovegrove, University of Hertfordshire, Hatfield, UK
Published online: May 2005
DOI: 10.1038/npg.els.0003812
Abstract
Western blotting (immunoblotting) is a system for the immunological detection of specific proteins that have been transferred
from a polyacrylamide gel to an immobilizing membrane. Proteins transferred to immobilizing filter membranes, such as nitrocellulose,
are well suited to high‐sensitivity solid‐phase immunoassay detection techniques.
Keywords: immunoblotting; electroblotting; immunodetection; electrophoresis
References
Beisiegel U
(1986)
Review: protein blotting.
Electrophoresis
7: 1–18.
Dunn S
(1986)
Effects of the modification of transfer buffer composition and the resaturation of proteins in the recognition of proteins on western blots by monoclonal antibodies.
Analytical Biochemistry
57: 144–153.
McKimm‐Breschkin JL
(1990)
The use of tetramethylbenzidine for solid phase immunoassays.
Journal of Immunological Methods
135: 277–280.
Miribel L and
Arnaud P
(1988)
Electrotransfer of proteins following polyacrylamide gel electrophoresis.
Journal of Immunological Methods
107: 253–259.
Myrseth LE,
Hagve TA and
Prydz H
(1989)
Separation and visualisation of Apolipoprotein B species by sodium dodecyl sulfate–agarose gel electrophoresis and immunoblotting.
Analytical Biochemistry
181: 86–89.
Thomas AS,
Ansford AJ and
Aaskov JG
(1989)
The application of immunoblotting to the phenotyping of group‐specific component.
Journal of Forensic Science Society
29: 197–205.
Towbin H,
Staehelin T and
Gordon J
(1979)
Electrophoretic transfer from polyacrylamide gels to nitrocellulose sheets: procedure & some applications.
Proceedings of the National Academy of Sciences of the USA
76: 4350–4354.