Western Blotting Analysis of Translation Products

Abstract

Western blotting (immunoblotting) is a system for the immunological detection of specific proteins that have been transferred from a polyacrylamide gel to an immobilizing membrane. Proteins transferred to immobilizing filter membranes, such as nitrocellulose, are well suited to high‐sensitivity solid‐phase immunoassay detection techniques.

Keywords: immunoblotting; electroblotting; immunodetection; electrophoresis

Figure 1.

Analysis of phosphoglucomutase (PGM)/β‐galactosidase fusion protein, expressed in Escherichia coli, after separation on an SDS gel. (a) Coomassie blue‐stained molecular weight markers. (b) Coomassie blue‐stained E. coli protein extract. (c) Immunostained western blot showing the PGM/β‐gal fusion protein in the same protein extract detected using sheep anti‐rabbit PGM primary antibodies.

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References

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How to Cite close
Whitehouse, D, and Lovegrove, J(May 2005) Western Blotting Analysis of Translation Products. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003812]