Posttranscriptional Processing

Abstract

Messenger ribonucleic acid (mRNA) is an intermediate template in the process of protein production from a deoxyribonucleic acid (DNA) template. It offers the cell the means to control the production and destination of proteins in a complex, timely and transient way, and increases greatly the potential variation of protein isoforms. The number of molecules of any one mRNA species in a mammalian cell can vary from less than 1 to as many as 10000. In human cells, an average of about six copies of each mRNA are present per cell.

Keywords: mRNA splicing; mRNA alternative splicing; mRNA editing; mRNA stability; mRNA transport; alternative polyadenylation site usage

References

Bashirullah A, Cooperstock RL and Lipshitz HD (2001) Spatial and temporal control of RNA stability. Proceedings of the National Academy of Sciences of the United States of America 98: 7025–7028.

Daneholt B (2001) Assembly and transport of a premessenger RNP particle. Proceedings of the National Academy of Sciences of the United States of America 98: 7012–7017.

Hastings ML and Krainer AR (2001) Pre‐mRNA splicing in the new millennium. Current Opinion in Cell Biology 13: 302–309.

Hertel KJ, Lynch KW and Maniatis T (1997) Common themes in the function of transcription and splicing enhancers. Current Opinion in Cell Biology 9: 350–357.

Keene JD (2001) Ribonucleoprotein infrastructure regulating the flow of genetic information between the genome and the proteome. Proceedings of the National Academy of Sciences of the United States of America 98: 7018–7024.

Keller W and MinvielleSebastia L (1997) A comparison of mammalian and yeast pre‐mRNA 3′‐end processing. Current Opinion in Cell Biology 9: 329–336.

Krecic AM and Swanson MS (1999) hnRNP complexes: composition, structure, and function. Current Opinion in Cell Biology 11: 363–371.

Maas S, Melcher T and Seeburg PH (1997) Mammalian RNA‐dependent deaminases and edited mRNAs. Current Opinion in Cell Biology 9: 343–349.

Mitchell P and Tollervey D (2001) mRNA turnover. Current Opinion in Cell Biology 13: 320–325.

Nakielny S and Dreyfuss G (1997) Nuclear export of proteins and RNAs. Current Opinion in Cell Biology 9: 420–429.

Nasmyth K and Jansen RP (1997) The cytoskeleton in mRNA localization and cell differentiation. Current Opinion in Cell Biology 9: 396–400.

Pestova TV, Kolupaeva VG, Lomakin IB, et al. (2001) Molecular mechanisms of translation initiation in eukaryotes. Proceedings of the National Academy of Sciences of the United States of America 98: 7029–7036.

Will CL and Luhrmann R (1997) Protein functions in pre‐mRNA splicing. Current Opinion in Cell Biology 9: 320–328.

Will CL and Luhrmann R (2001) Spliceosomal UsnRNP biogenesis, structure and function. Current Opinion in Cell Biology 13: 290–301.

Further Reading

Bass BL (2001) RNA editing. Frontiers in Molecular Biology 34.

Belasco J and Brawerman G (1997) Control of Messenger RNA Stability. London, UK: Academic Press.

Brennan CM and Steitz JA (2001) HuR and mRNA stability. Cellular and Molecular Life Sciences 58: 266–277.

Bussell K (2001) A brief history of the nucleus. Nature Reviews Molecular Cell Biology 2: 407.

Dandekar T and Bengert P (2001) RNA Motifs and Regulatory Elements. New York, NY: Springer‐Verlag.

Gesteland RF, Cech TR and Atkins JF (2000) The RNA World, Monograph 37. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

Morris DR (1997) mRNA metabolism and posttranscriptional gene regulation. In: Harford JB (ed) Modern Cell Biology, vol. 117. New York, NY: Wiley‐Liss.

Krainer AR (1997) Eukaryotic mRNA processing. Frontiers in Molecular Biology 17.

Lamond AI (1996) Pre‐mRNA Processing. New York, NY: Springer‐Verlag.

Lewis JD and Tollervey D (2000) Like attracts like: getting RNA processing together in the nucleus. Science 288: 1385–1389.

Richter JD (1997) mRNA Formation and Function. London, UK: Academic Press.

Shatkin AJ and Manley JL (2000) The ends of the affair: capping and polyadenylation. Nature Structural Biology 7: 838–842.

Siomi MC (2000) The molecular mechanisms of messenger RNA nuclear export. Cell Structure and Function 25: 227–235.

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How to Cite close
Hodges, Angela Kaye(Sep 2005) Posttranscriptional Processing. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005287]