Recombinant DNA

Abstract

Recombinant deoxyribonucleic acid (DNA) is that which has been created by cutting and joining naturally occurring or synthetic DNA, producing molecules unlike those seen in nature. Recombinant molecules are most commonly made as a means of propagating an isolated fragment of DNA in bacteria, to obtain large amounts of the fragment for sequencing or other purposes.

Keywords: cloning; restriction endonuclease; ligation; vector; transformation; transfection

Further Reading

Primrose SB, Twyman RM and Old RW (2002) Principles of Gene Manipulation. Oxford, UK: Blackwell Science.

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How to Cite close
Dear, Paul H(Sep 2005) Recombinant DNA. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005329]