Burkhard Tümmler, Medizinische Hochschule Hannover, Hannover, Germany
Frauke Mekus, Medizinische Hochschule Hannover, Hannover, Germany
Published online: September 2005
DOI: 10.1038/npg.els.0005330
Genomic DNA can be prepared from any source by three steps: cell lysis, deproteinization and recovery of DNA. The basic protocol needs to be adapted to the demands of the application, the requested yield, purity and molecular weight of the DNA, and the amount and history of the source.
Keywords: agarose-embedded DNA; ancient DNA; forensic medicine; paleontology; high molecular weight DNA
| References | |
| book Ausubel FM, Brent R, Kingston RE, et al. (eds.) (1997) Current Protocols in Molecular Biology. New York, NY: John Wiley & Sons. | |
| Bachmann L, Scholz M, Broghammer M, Giddings I and Pusch CM (2000) Voltage-induced release of nucleic acids from palaeontological samples. Electrophoresis 21: 1488–1492. | |
| book Bautsch W, Römling U, Schmidt KD, et al. (1997) "Long-range restriction mapping of genomic DNA". In: Dear PH (ed.) Genome Mapping – A Practical Approach, pp. 281–313. Oxford, UK: Oxford University Press. | |
| Cattaneo C, Craig OE, James NT and Sokol RJ (1997) Comparison of three extraction methods on bone and blood stains up to 43 years old and amplification of three different gene sequences. Journal of Forensic Sciences 42: 1126–1135. | |
| Goldberg JB and Ohman DE (1984) Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate. Journal of Bacteriology 158: 1115–1121. | |
| Gross-Bellard M, Oudet P and Chambon P (1973) Isolation of high-molecular-weight DNA from mammalian cells. European Journal of Biochemistry 36: 32–38. | |
| Kalmar T, Bachrati CZ, Marcsik A and Rasko I (2000) A simple and efficient method for PCR amplifiable DNA extraction from ancient bones. Nucleic Acids Research 28: E67. | |
| Klintschar M and Neuhuber F (2000) Evaluation of an alkaline lysis method for the extraction of DNA from whole blood and forensic stains for STR analysis. Journal of Forensic Sciences 45: 669–673. | |
| Lambert DM, Ritchie PA, Millar CD, et al. (2002) Rates of evolution in ancient DNA from Adelie penguins. Science 295: 2270–2273. | |
| Murray MG and Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Research 8: 4321–4325. | |
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