Restriction Enzymes

Mala Mani, Johns Hopkins University, Baltimore, Maryland, USA
Karthikeyan Kandavelou, Johns Hopkins University, Baltimore, Maryland, USA
Joy Wu, Johns Hopkins University, Baltimore, Maryland, USA
Srinivasan Chandrasegaran, Johns Hopkins University, Baltimore, Maryland, USA

Published online: September 2005

DOI: 10.1038/npg.els.0005332

Restriction enzymes have played a key role in the completion of the initial sequencing and analysis of the first draft of the human genome. They were important tools in the cloning, analysis and sequencing of the various deoxyribonucleic acid fragments as well as in the construction of a physical map of the human genome.

Keywords: restriction enzymes; endonucleases; modification enzymes; methylases; restriction–modification system; restriction mapping; recombinant DNA

 References
    book Ausubel FN, Brent R, Kingston RE, et al. (eds.) (2001) "Enzymatic manipulation of DNA and RNA". In: Current Protocols in Molecular Biology, vol. 1, chap. 3, pp. 3.0.1–3.19.8, New York, NY: John Wiley.
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    book New England Biolabs (2000–2001) New England Biolabs Catalog and Technical Reference, 2000–2001. Beverly, MA: New England Biolabs. (See the appendix for detailed information about restriction–modification enzymes.)
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 Further Reading
    Aggarwal AK and Wah DA (1998) Novel site-specific DNA endonuclease. Current Opinions in Structural Biology 8(1): 19–25.
    book Chandrasegaran S (2001) Restriction enzymes. Encyclopedia of Life Sciences, http://www.els.net. London, UK: Nature Publishing Group.
    book Chandrasegaran S and Reddy S (2001) "Ligation: theory and practice". Encyclopedia of Life Sciences, http://www.els.net. London, UK: Nature Publishing Group.
    Chandrasegaran S and Smith J (1999) Chimeric restriction enzymes: what is next? Biological Chemistry 380: 841–848.
    book Gormley NA, Watson MA and Halford SE (2001) Bacterial restriction–modification systems. Encyclopedia of Life Sciences, http://www.els.net. London, UK: Nature Publishing Group.
    Klug A (1999) Zinc finger peptides for the regulation of gene expression. Journal of Molecular Biology 293: 215–218.
    Pabo CO, Peisach E and Grant A (2001) Design and selection of novel Cys2 His2 zinc finger proteins. Annual Review of Biochemistry 70: 313–340.
    Pingoud A and Jeltsch A (2001) Structure and function of type II restriction endonuclease. Nucleic Acids Research 29: 3705–3727.
    Roberts RJ and Cheng X (1998) Base flipping. Annual Review of Biochemistry 67: 181–198.
    book Roberts RJ and Hulford SE (1993) "Type II restriction endonuclease". In: Linn SM, Lloyd RS and Roberts RJ (eds.) Nuclease, pp. 35–38. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

Related Sub-Topics:

Nucleic Acid Structure | Replication and Recombination | Chromosomes and Chromatin Modifications | Techniques and Tools in Molecular Biology | DNA Damage and Repair | Enzymes: Structure and Action Mechanism | Nucleic Acids: Structure, Function, Metabolism
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Article Title: Restriction Enzymes
 
How to Cite close
Mani, Mala, Kandavelou, Karthikeyan, Wu, Joy, and Chandrasegaran, Srinivasan(Sep 2005) Restriction Enzymes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005332]