Robotics and Automation in Molecular Genetics

Trevor Hawkins, US DOE Joint Genome Institute, Walnut Creek, California, USA
Chris Elkin, US DOE Joint Genome Institute, Walnut Creek, California, USA
Martin Pollard, US DOE Joint Genome Institute, Walnut Creek, California, USA

Published online: September 2005

DOI: 10.1038/npg.els.0005351

Robotic platforms and automation can be applied to laboratory molecular biology protocols to achieve high-throughput sample processing for DNA sequencing, genomics, proteomics and other biological applications.

Keywords: human genome project; automation; robotics; DNA sequencing

Figure 1. Microchannel electrophoresis chip used for separation and detection of labeled deoxyribonucleic acid (DNA) sequencing fragments. This 16-channel chip was fabricated at Molecular Dynamics, Sunnyvale, CA, by Dr Stevan Jovanovich.
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 Further Reading
    Deamer DW and Akeson M (2000) Nanopores and nucleic acids: prospects for ultrarapid sequencing. Trends in Biotechnology 18: 147–151.
    Dolnik V, Liu S and Jovanovich S (2000) Capillary electrophoresis on microchips. Electrophoresis 21: 41–54.
    Kheterpal I, Scherer JR, Clark SM, et al. (1996) DNA sequencing using a four-color confocal fluorescence capillary array scanner. Electrophoresis 17: 1852–1859.
    Marziali A and Akeson M (2001) New DNA sequencing methods. Annual Review of Biomedical Engineering 3: 195–223.
    Meldrum D (2000) Automation for genomics, part II: sequencers, microarrays, and future trends. Genome Research 10: 1288–1303.
    Medintz IL, Paegel BM and Mathies RA (2001) Microfabricated capillary array electrophoresis DNA analysis systems. Journal of Chromatography A 924: 265–270.
    Paegel BM, Hutt LD, Simpson PC and Mathies RA (2000) Turn geometry for minimizing band broadening in microfabricated capillary electrophoresis channels. Analytical Chemistry 72: 3030–3037.
    Rief M, Clausen-Schaumann H and Gaub HE (1999) Sequence-dependent mechanics of single DNA molecules. Nature Structural Biology 6: 346–349.
    Schmalzing D, Koutny L, Salas-Solano O, et al. (1999) Recent developments in DNA sequencing by capillary and microdevice electrophoresis. Electrophoresis 20: 3066–3077.
    Zhou H, Miller AW, Sosic Z, et al. (2000) DNA sequencing up to 1300 bases in two hours by capillary electrophoresis with mixed replaceable linear polyacrylamide solutions. Analytical Chemistry 72: 1045–1052.

Related Sub-Topics:

Replication and Recombination | Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology
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Article Title: Robotics and Automation in Molecular Genetics
 
How to Cite close
Hawkins, Trevor, Elkin, Chris, and Pollard, Martin(Sep 2005) Robotics and Automation in Molecular Genetics. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005351]