Radiation Hybrid Mapping

Abstract

Radiation hybrid mapping is a method that combines aspects of both genetic and physical mapping and is extensively used in the construction of whole‐genome maps. A panel of radiation hybrid cell lines, each retaining a different portion of the donor genome in the background of a recipient cell, is used to score the presence or absence of sequence tagged sites.

Keywords: radiation hybrids; panels; mapping; map; genome

Figure 1.

Schematic drawing illustrating the main steps in the construction of radiation hybrid (RH) panels. The parameters used in the construction of the Genebridge 4 (Gyapay et al., ) panel are given as an example. The human diploid fibroblast cell line HFL121 (donor) is irradiated with 3000 rad and fused to A23 TK (deficient in thymidine kinase) hamster cells (recipient). The hybrids are selected in hypoxanthine–aminopterin–thymidine (HAT) medium on which the hamster cells that have not retained a fragment harboring the human TK gene are unable to grow. The irradiation dose is lethal to the human cells. The donor DNA fragments (white bars) are retained as extra chromosomes or they integrate into the hamster chromosomes (black bars). Hybrids that form individual colonies are picked and further characterized using a small set of genome‐wide markers. Typically, on the basis of their retention characteristics a set of ca. 100 hybrids is assembled into the mapping panel. PEG: polyethylene glycol.

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Web Links

PRIMER version 3 http://www‐genome.wi.mit.edu/cgi‐bin/primer/primer3_www.cgi

REPEATMASKERhttp://www.genome.washington.edu/uwgc/analysistools/repeatmask.htm

MULTIMAP http://linkage.rockefeller.edu/multimap

RHMAP http://www.sph.umich.edu/group/statgen

RHMAPPER http://www.genome.wi.mit.edu/ftp/pub/software/rhmapper

SAMAPPER http://shgc.stanford.edu

Additional software tools http://compgen.rutgers.edu/rhmap/#programs/

Research Genetics http://www.resgen.com/intro/mapping.php3

WGRH panels http://compgen.rutgers.edu/rhmap

RHdb. Source of RH vectors, hosted by the European Bioinformatics Institute http://www.ebi.ac.uk:80/RHdb/

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How to Cite close
Deloukas, Panos(Sep 2005) Radiation Hybrid Mapping. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005361]