Radiation Hybrid Mapping

Panos Deloukas, The Wellcome Trust Sanger Institute, Cambridge, UK

Published online: September 2005

DOI: 10.1038/npg.els.0005361

Radiation hybrid mapping is a method that combines aspects of both genetic and physical mapping and is extensively used in the construction of whole-genome maps. A panel of radiation hybrid cell lines, each retaining a different portion of the donor genome in the background of a recipient cell, is used to score the presence or absence of sequence tagged sites.

Keywords: radiation hybrids; panels; mapping; map; genome

Figure 1. Schematic drawing illustrating the main steps in the construction of radiation hybrid (RH) panels. The parameters used in the construction of the Genebridge 4 (Gyapay et al., 1996) panel are given as an example. The human diploid fibroblast cell line HFL121 (donor) is irradiated with 3000 rad and fused to A23 TK– (deficient in thymidine kinase) hamster cells (recipient). The hybrids are selected in hypoxanthine–aminopterin–thymidine (HAT) medium on which the hamster cells that have not retained a fragment harboring the human TK gene are unable to grow. The irradiation dose is lethal to the human cells. The donor DNA fragments (white bars) are retained as extra chromosomes or they integrate into the hamster chromosomes (black bars). Hybrids that form individual colonies are picked and further characterized using a small set of genome-wide markers. Typically, on the basis of their retention characteristics a set of ca. 100 hybrids is assembled into the mapping panel. PEG: polyethylene glycol.
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 Web Links
    ePath PRIMER version 3 http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi
    ePath REPEATMASKERhttp://www.genome.washington.edu/uwgc/analysistools/repeatmask.htm
    ePath MULTIMAP http://linkage.rockefeller.edu/multimap
    ePath RHMAP http://www.sph.umich.edu/group/statgen
    ePath RHMAPPER http://www.genome.wi.mit.edu/ftp/pub/software/rhmapper
    ePath SAMAPPER http://shgc.stanford.edu
    ePath Additional software tools http://compgen.rutgers.edu/rhmap/#programs/
    ePath Research Genetics http://www.resgen.com/intro/mapping.php3
    ePath WGRH panels http://compgen.rutgers.edu/rhmap
    ePath RHdb. Source of RH vectors, hosted by the European Bioinformatics Institute http://www.ebi.ac.uk:80/RHdb/

Related Sub-Topics:

Techniques in Cell Biology | Techniques and Tools in Clinical Genetics | Gene Expression Profiling of Genetic Disease | Techniques and Tools in Molecular Biology
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Article Title: Radiation Hybrid Mapping
 
How to Cite close
Deloukas, Panos(Sep 2005) Radiation Hybrid Mapping. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005361]