Restriction Mapping

Burkhard Tümmler, Medizinische Hochschule Hannover, Hannover, Germany
Frauke Mekus, Medizinische Hochschule Hannover, Hannover, Germany

Published online: September 2005

DOI: 10.1038/npg.els.0005363

Deoxyribonucleic acid is partially or completely digested with one or more restriction endonucleases. The generated fragments are separated by size, and then ordered by combinatorial analysis to generate a physical map.

Keywords: agarose gel electrophoresis; physical map; pulsed-field gel electrophoresis; restriction endonuclease; southern blot hybridization

 Further Reading
    book Bautsch W, Römling U, Schmidt KD, et al. (1997) "Long-range restriction mapping of genomic DNA". In: Dear PH (ed.) Genome Mapping – A Practical Approach, pp. 281–313. Oxford, UK: IRL/Oxford University Press.
    Boseley PG, Moss T and Birnstiel ML (1980) 5¢ Labelling and poly(dA) tailing. Methods in Enzymology 65: 478–494.
    Danna AJ (1980) Determination of fragment order through partial digests and multiple enzyme digests. Methods in Enzymology 65: 449–467.
    book Römling U, Fislage R and Tümmler B (1996) "Macrorestriction mapping and analysis of bacterial genomes". In: Birren B and Lai E (eds.) Nonmammalian Genome Analysis, pp. 165–195. San Diego, CA: Academic Press.

Related Sub-Topics:

Techniques and Tools in Clinical Genetics | Techniques and Tools in Molecular Biology | Enzymes: Structure and Action Mechanism | Nucleic Acids: Structure, Function, Metabolism
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Article Title: Restriction Mapping
 
How to Cite close
Tümmler, Burkhard, and Mekus, Frauke(Sep 2005) Restriction Mapping. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005363]