Restriction Mapping

Abstract

Deoxyribonucleic acid is partially or completely digested with one or more restriction endonucleases. The generated fragments are separated by size, and then ordered by combinatorial analysis to generate a physical map.

Keywords: agarose gel electrophoresis; physical map; pulsed‐field gel electrophoresis; restriction endonuclease; southern blot hybridization

Further Reading

Bautsch W, Römling U, Schmidt KD, et al. (1997) Long‐range restriction mapping of genomic DNA. In: Dear PH (ed.) Genome Mapping – A Practical Approach, pp. 281–313. Oxford, UK: IRL/Oxford University Press.

Boseley PG, Moss T and Birnstiel ML (1980) 5′ Labelling and poly(dA) tailing. Methods in Enzymology 65: 478–494.

Danna AJ (1980) Determination of fragment order through partial digests and multiple enzyme digests. Methods in Enzymology 65: 449–467.

Römling U, Fislage R and Tümmler B (1996) Macrorestriction mapping and analysis of bacterial genomes. In: Birren B and Lai E (eds.) Nonmammalian Genome Analysis, pp. 165–195. San Diego, CA: Academic Press.

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How to Cite close
Tümmler, Burkhard, and Mekus, Frauke(Sep 2005) Restriction Mapping. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005363]