Caenorhabditis elegans Genome Project


The sequence of the 100‐Mb genome of the nematode Caenorhabditis elegans, the first sequence of a multicellular organism, was published in 1998. The methodologies and strategies developed for the worm project, as well as the philosophy of continuous and immediate data release, have carried through to the Human Genome Project (HGP).

Keywords: Caenorhabditis elegans; genome; sequence; annotation

Figure 1.

Physical map (Pmap) of a region in the Caenorhabditis elegans genome showing the tiling path of yeast artificial chromosome (YAC) clones (upper region A), cosmid/fosmid mapping (middle region B) and mapped genetic loci (bottom region C).

Figure 2.

Schema for the analysis pipeline used in the analysis of the C. elegans genome sequence. Finished sequence from the sequence editors GAP4 and CONSED is subjected to a number of computational algorithms (similarity searches, repeat matches and gene predictions). Data are consolidated in the ACEDB database and evaluated by an annotator. The sequence with its annotations is then submitted to the public nucleotide databases (EMBL/GenBank).

Figure 3.

Sequence display (Fmap) for a region of the C. elegans genome showing two gene predictions. Coding exons, connected by introns, are displayed to the right of the scale. The extent of transcript data is indicated by the rectangles to the right of the gene prediction. Similarity data are displayed in the next columns, reading from left to right: C. elegans expressed sequence tag, worm peptide, fly peptide, yeast peptide, human peptide, other peptides and C. briggsae genomic matches. The two columns on the far right indicate the location of repeats in this sequence region.



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Further Reading

Ambros V (2001) MicroRNAs: tiny regulators with great potential. Cell 107: 823–826.

Caenorhabditis elegans Sequencing Consortium (1998) Genome sequence of the nematode C. elegans: a platform for investigating biology. Science 282: 2012–2018.

Caenorhabditis elegans Sequencing Consortium (1999) How the worm was won. Trends in Genetics: TIG 15: 51–58.

Hodgkin J and Herman RK (1998) Changing styles in C. elegans genetics. Trends in Genetics 149: 352–357.

Hope IA (ed.) (1999) Caenorhabditis elegans: A Practical Approach. New York: Oxford University Press.

Kim SK (2001) Caenorhabditis elegans: mining the functional genomic landscape. Nature Reviews Genetics 2: 681–689.

Riddle DL, Blumenthal T, Meyer BJ and Priess JR (eds.) (1997) Caenorhabditis elegans II. Plainview, NY: Cold Spring Harbor Laboratory Press.

Web Links

ACEDB. Developed in 1989 by J. Thierry‐Mieg and, R. Durbin for the C. elegans genome project

EMBL‐EBI. European Bioinformatics Institute. EMBL nucleotide sequence database

GenBank Database. National Institutes of Health genetic sequence database genbank.html

WormBase. Genome and Biology of C. elegans

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How to Cite close
Coulson, Alan, and Lawson, Daniel(Sep 2005) Caenorhabditis elegans Genome Project. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0005368]