Expression Analysis In Vivo

Rudi Balling, Gesellschaft für Biotechnologische Forschung (GBF), Braunschweig, Germany
Werner Müller, Mascheroder Weg 1, Braunschweig, Germany

Published online: September 2006

DOI: 10.1002/9780470015902.a0005682

Analyzing gene expression in vivo is an unresolved technical task. Finding ways to see genes expressed without disturbing a complex organism is best done by inserting easily detectable marker genes into the genome, by replacing one copy of the endogenous gene and subsequently observing its expression under the microscope or through very sensitive CCD cameras.

Keywords: in vivo imaging; homologous recombination; marker genes; green fluorescence protein; GFP; luciferase

 References
    Giaever G, Chu AM, Ni L, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418: 387–391.
    Goffeau A, Barrell BG, Bussey H, et al. (1996) Life with 6000 genes. Science 274: 563–567.
    Hazan MT, Schönig K, Berger S, Graewe W and Bujard H (2001) Long-term, non-invasive imaging of regulated gene expression in living mice. Genesis 29: 116–122.
    Schreiber G, Tsykin A, Aldred AR, et al. (1989) The acute phase response in the rodent. Annals of the New York Academy of Sciences 557: 61–85.
    Vooijs M, Jonkers J, Lyons S and Berns A (2002) Noninvasive imaging of spontaneous retinoblastoma pathway-dependent tumors in mice. Cancer Research 62: 1862–1867.
    Wu JC, Sundaresan G, Iyer M and Gambhir SS (2001) Noninvasive optical imaging of firefly luciferase reporter gene expression in skeletal muscles of living mice. Molecular Therapy 4: 297–306.
 Further Reading
    book Nagy A (ed.) (1998) Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory.
    book Joyner AL (ed.) (2000) Gene Targeting: A Practical Approach. Oxford: Oxford University Press.
    book Simpson RJ (ed.) (1999) Proteins and Proteomics: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory.
    book Westermeier R and Naven T (2001) Proteomics in Practice: A Laboratory Manual of Proteome Analysis. New York: John Wiley.
 Web Links
    ePath BD Biosciences Clontech – References. Incorporating the Tet system in transgenic organisms http://www.clontech.com/tet/Refs/TetRefstrans.shtml
    ePath Biology of the Mammary Gland. Animal models to study mammary gland development, physiology, and tumorigenesis http://mammary.nih.gov/models/index.html
    ePath Chemistry 2002. The Nobel Prize in Chemistry 2002 http://www.nobel.se/chemistry/laureates/2002/index.html
    ePath CLONTECHniques Archives. Generation of GFP transgenic mice http://www.clontech.com/archive/JAN96UPD/MouseGFParticle.shtml
    ePath Life with 6000 genes. This link points to the original paper describing the genome of Saccharomyces cerevisiae http://www.sciencemag.org/cgi/content/full/274/5287/546
    ePath Nagy Lab. Samuel Lunenfeld Research Institute. This link points to the database of cre-transgenic mouse lines, maintained by the Nagy Lab http://www.mshri.on.ca/nagy/cre.htm
    ePath Saccharomyces Genome Database http://genome-www.stanford.edu/Saccharomyces/
    ePath Xenogen Technology Publications. Bibliography http://www.xenogen.com/pubs.html

Related Sub-Topics:

Techniques in Cell Biology | Techniques and Tools in Clinical Genetics | Gene Expression Profiling of Genetic Disease | RNA Structure and Function | Techniques and Tools in Molecular Biology | Computational Molecular Biology
Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

Article Title: Expression Analysis In Vivo
 
How to Cite close
Balling, Rudi, and Müller, Werner(Sep 2006) Expression Analysis In Vivo. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0005682]