Expression Analysis In Vivo

Abstract

Analyzing gene expression in vivo is an unresolved technical task. Finding ways to see genes expressed without disturbing a complex organism is best done by inserting easily detectable marker genes into the genome, by replacing one copy of the endogenous gene and subsequently observing its expression under the microscope or through very sensitive CCD cameras.

Keywords: in vivo imaging; homologous recombination; marker genes; green fluorescence protein; GFP; luciferase

References

Giaever G, Chu AM, Ni L, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418: 387–391.

Goffeau A, Barrell BG, Bussey H, et al. (1996) Life with 6000 genes. Science 274: 563–567.

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Further Reading

Nagy A (ed.) (1998) Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory.

Joyner AL (ed.) (2000) Gene Targeting: A Practical Approach. Oxford: Oxford University Press.

Simpson RJ (ed.) (1999) Proteins and Proteomics: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory.

Westermeier R and Naven T (2001) Proteomics in Practice: A Laboratory Manual of Proteome Analysis. New York: John Wiley.

Web Links

BD Biosciences Clontech – References. Incorporating the Tet system in transgenic organisms http://www.clontech.com/tet/Refs/TetRefstrans.shtml

Biology of the Mammary Gland. Animal models to study mammary gland development, physiology, and tumorigenesis http://mammary.nih.gov/models/index.html

Chemistry 2002. The Nobel Prize in Chemistry 2002 http://www.nobel.se/chemistry/laureates/2002/index.html

CLONTECHniques Archives. Generation of GFP transgenic mice http://www.clontech.com/archive/JAN96UPD/MouseGFParticle.shtml

Life with 6000 genes. This link points to the original paper describing the genome of Saccharomyces cerevisiae http://www.sciencemag.org/cgi/content/full/274/5287/546

Nagy Lab. Samuel Lunenfeld Research Institute. This link points to the database of cre‐transgenic mouse lines, maintained by the Nagy Lab http://www.mshri.on.ca/nagy/cre.htm

Saccharomyces Genome Database http://genome‐www.stanford.edu/Saccharomyces/

Xenogen Technology Publications. Bibliography http://www.xenogen.com/pubs.html

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How to Cite close
Balling, Rudi, and Müller, Werner(Sep 2006) Expression Analysis In Vivo. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0005682]