Naked DNA in Gene Therapy

Abstract

‘Naked DNA’ is DNA without an association with any synthetic or naturally occurring compounds. Significant progress has been made in the use of naked DNA for gene delivery.

Keywords: electroporation; gene therapy; hydrodynamic DNA delivery; naked DNA; nonviral vector

References

Buchman AR and Berg P (1988) Comparison of intron‐dependent and intron‐independent gene expression. Molecular and Cellular Biology 8: 4395–4405.

Ferber D (2001) Gene therapy: safer and virus‐free? Science 294: 1638–1642.

Fewell JG, MacLaughlin F, Mehta V, et al. (2001) Gene therapy for the treatment of hemophilia B using PINC‐formulated plasmid delivered to muscle with electroporation. Molecular Therapy 3: 574–583.

Kishida T, Asada H, Satoh E, et al. (2001) In vivo electroporation‐mediated transfer of interleukin‐12 and interleukin‐18 genes induces significant antitumor effects against melanoma in mice. Gene Therapy 8: 1234–1240.

Liu F, Song Y and Liu D (1999) Hydrodynamics‐based transfection in animals by systemic administration of plasmid DNA. Gene Therapy 6: 1258–1266.

Miao CH, Thompson AR, Loeb K and Ye X (2001) Long‐term and therapeutic‐level hepatic gene expression of human factor IX after naked plasmid transfer in vivo. Molecular Therapy 3: 947–957.

Neumann E, Schaefer‐Ridder M, Wang Y and Hofschneider PH (1982) Gene transfer into mouse lyoma cells by electroporation in high electric fields. The EMBO Journal 1: 841–845.

Stoll SM, Sclimenti CR, Baba EJ, et al. (2001) Epstein–Barr virus/human vector provides high‐level, long‐term expression of alpha‐1 antitrypsin in mice. Molecular Therapy 4: 122–129.

Wang R, Doolan DL, Le TP, et al. (1998) Induction of antigen‐specific cytotoxic T lymphocytes in humans by a malaria DNA vaccine. Science 282: 476–480.

Wolff JA, Malone RW, Williams P, et al. (1990) Direct gene transfer into mouse muscle in vivo. Science 247: 1465–1468.

Zhang G, Budker V and Wolff JA (1999) High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA. Human Gene Therapy 10: 1735–1737.

Zhang G, Song YK and Liu D (2000) Long‐term expression of human alpha‐1‐antitrypsin gene in mouse liver achieved by intravenous administration of plasmid DNA using a hydrodynamics‐based procedure. Gene Therapy 7: 1344–1349.

Further Reading

Huang L, Wagner E and Hung MC (1999) Nonviral Vectors for Gene Therapy. San Diego, CA: Academic Press.

Jaroszeski MJ and Malone RW (2000) Theory and in vivo application of electroporative gene delivery. Molecular Therapy 2: 178–187.

Liu D and Knapp JE (2001) Hydrodynamics‐based gene delivery. Current Opinion in Molecular Therapeutics 3: 192–197.

Liu MA and Ulmer JB (2000) Gene‐based vaccines. Molecular Therapy 6: 497–500.

Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.

Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

How to Cite close
Liu, Dexi, Satoh, Etsuko, and Knapp, Joseph E(Jan 2006) Naked DNA in Gene Therapy. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005744]