Nigel P Carter, The Sanger Centre, Cambridge, UK
Published online: January 2006
DOI: 10.1038/npg.els.0005782
Using flow sorting, metaphase chromosomes can be separated into purified fractions that can be used for constructing chromosome-specific DNA libraries, chromosome painting probes and can be used as targets for PCR-based gene mapping and sequencing.
Keywords: chromosomes; flow cytometry; flow-sorting; karyotype; chromosome painting
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Figure 1. Bivariate flow karyotype of a lymphoblastoid cell line derived from a normal male.
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Figure 2. Bivariate flow karyotype of a cell line derived from an individual with a balanced translocation between chromosomes 9 and 22. The derivative chromosomes are different in size from the normal homologs and so can be resolved easily.
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Figure 3. Spectral karyotype of a normal male metaphase. This image was produced by the participants of the 28th Wellcome Trust Advanced Course held at the Sanger Centre, March 2001.
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