Flow‐sorted Chromosomes

Abstract

Using flow sorting, metaphase chromosomes can be separated into purified fractions that can be used for constructing chromosome‐specific DNA libraries, chromosome painting probes and can be used as targets for PCR‐based gene mapping and sequencing.

Keywords: chromosomes; flow cytometry; flow‐sorting; karyotype; chromosome painting

Figure 1.

Bivariate flow karyotype of a lymphoblastoid cell line derived from a normal male.

Figure 2.

Bivariate flow karyotype of a cell line derived from an individual with a balanced translocation between chromosomes 9 and 22. The derivative chromosomes are different in size from the normal homologs and so can be resolved easily.

Figure 3.

Spectral karyotype of a normal male metaphase. This image was produced by the participants of the 28th Wellcome Trust Advanced Course held at the Sanger Centre, March 2001.

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References

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How to Cite close
Carter, Nigel P(Jan 2006) Flow‐sorted Chromosomes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005782]