Flow‐sorted Chromosomes


Using flow sorting, metaphase chromosomes can be separated into purified fractions that can be used for constructing chromosome‐specific DNA libraries, chromosome painting probes and can be used as targets for PCR‐based gene mapping and sequencing.

Keywords: chromosomes; flow cytometry; flow‐sorting; karyotype; chromosome painting

Figure 1.

Bivariate flow karyotype of a lymphoblastoid cell line derived from a normal male.

Figure 2.

Bivariate flow karyotype of a cell line derived from an individual with a balanced translocation between chromosomes 9 and 22. The derivative chromosomes are different in size from the normal homologs and so can be resolved easily.

Figure 3.

Spectral karyotype of a normal male metaphase. This image was produced by the participants of the 28th Wellcome Trust Advanced Course held at the Sanger Centre, March 2001.



Carter NP (1994a) Bivariate chromosome analysis using a commercial flow cytometer. Methods in Molecular Biology 29: 187–204.

Carter NP (1994b) Cytogenetic analysis by chromosome painting. Cytometry 18: 2–10.

Carter NP, Ferguson‐Smith MA, Perryman MT, et al. (1992) Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics. Journal of Medical Genetics 29: 299–307.

van den Engh GJ, Trask BJ, Gray JW, Langlois RG and Yu LC (1985) Preparation and bivariate analysis of suspensions of human chromosomes. Cytometry 6: 92–100.

Langford CF, Fischer PE, Binns MM, Holmes NG and Carter NP (1996) Chromosome‐specific paints from a high‐resolution flow karyotype of the dog. Chromosome Research 4: 115–123.

Lucretti S and Dolezel J (1997) Bivariate flow karyotyping in broad bean (Vicia faba). Cytometry 28: 236–242.

Ross MT and Langford CF (1997) The use of flow‐sorted chromosomes in genome mapping. In: Dear PH (ed.) Genome Mapping: A Practical Approach, pp. 165–182. New York, NY: IRL Press at Oxford University Press.

Schrock E, du Manoir S, Veldman T, et al. (1996) Multicolor spectral karyotyping of human chromosomes. Science 273: 494–497.

Sillar R and Young BD (1981) A new method for the preparation of metaphase chromosomes for flow analysis. Journal of Histochemistry and Cytochemistry 29: 74–78.

Speicher MR, Gwyn Ballard S and Ward DC (1996) Karyotyping human chromosomes by combinatorial multi‐fluor FISH. Nature Genetics 12: 368–375.

Telenius H, Carter NP, Bebb CE, et al. (1992a) Degenerate oligonucleotide‐primed PCR: general amplification of target DNA by a single degenerate primer. Genomics 13: 718–725.

Telenius H, Pelmear AH, Tunnacliffe A, et al. (1992b) Cytogenetic analysis by chromosome painting using DOP‐PCR amplified flow‐sorted chromosomes. Genes Chromosomes Cancer 4: 257–263.

Trask B, van den Engh G and Gray JW (1989) Inheritance of chromosome heteromorphisms analyzed by high‐resolution bivariate flow karyotyping. American Journal of Human Genetics 45: 753–760.

Trask B, van den Engh G, Nussbaum R, Schwartz C and Gray J (1990) Quantification of the DNA content of structurally abnormal X chromosomes and X chromosome aneuploidy using high resolution bivariate flow karyotyping. Cytometry 11: 184–195.

Van Dilla MA, Deavan LL, Albright KL, et al. (1989) The National Laboratory Gene Library Project. In: Gray JW (ed.) Flow Cytogenetics, pp. 257–274. London, UK: Academic Press.

Wienberg J and Stanyon R (1997) Comparative painting of mammalian chromosomes. Current Opinion in Genetics and Development 7: 784–791.

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How to Cite close
Carter, Nigel P(Jan 2006) Flow‐sorted Chromosomes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005782]