Comparative Cytogenetics

In the pre-genomics era, the field of comparative cytogenetics was restricted to the comparison of banded chromosome preparations of the species being studied. The development of molecular biology and subsequent convergence of this field with conventional cytogenetics led to the development of molecular cytogenetics. The application of molecular cytogenetics, in particular multicolour fluorescence in situ hybridization (MFISH), has revolutionized our ability to compare genomes at the chromosomal, subchromosomal and molecular levels, leading to much more defined knowledge about the processes of chromosomal rearrangement that have occurred during evolution. In this article we review very briefly some of the work that has led to a greater understanding of the comparative cytogenetics of mammals, in the context of their relationship to the human karyotype.

Keywords: chromosomes; karyotype evolution; molecular cytogenetics; rearrangement; Zoo-FISH

Figure 1. 4,6-Diamidino-2-phenylindole (DAPI)-banded karyotype of the domestic dog, Canis familiaris.
Figure 2. Comparative chromosome painting (Zoo-FISH) images visualizing evolutionarily conserved chromosome segments (ECCS) between human and dog genomes: (a) human chromosome 1 paint is hybridized to human metaphase chromosomes; (b) the same human chromosome 1 paint is hybridized to dog metaphase chromosomes.
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 References
    book Benton MJ (1990) Vertebrate Palaeontology: Biology and Evolution. London, UK: Harper Collins.
    Breen M, Hitte C, Lorentzen TD et al. (2004) An integrated 4249 marker FISH/RH map of the canine genome. BMC Genomics 5: 65.
    Breen M, Thomas R, Binns MM, Carter NP and Langford CF (1999) Reciprocal chromosome painting reveals detailed regions of conserved synteny between the karyotypes of the domestic dog (Canis familiaris) and human. Genomics 61: 145–155.
    Carbone L, Vessere GM, Boudewijn FH et al. (2006) A high resolution map of synteny disruptions in gibbon and human genomes. PLoS Genetics 2: 2163–2175.
    Chowdhary BP, Fronicke L, Gustavsson I and Scherthan H (1996) Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based chromosomal homologies. Mammalian Genome 7: 297–302.
    Consortium IHGS (2001) Initial sequencing and analysis of the human genome. Nature 409: 860–921.
    Duke SE, Samollow PB, Mauceli E, Lindblad-Toh K and Breen M (2007) Integrated cytogenetic BAC map of the genome of the gray, short-tailed opossum, Monodelphis domestica. Chromosome Research 15: 361–370.
    Evans HJ, Buckland RA and Sumner AT (1973) Chromosome homology and heterochromatin in goat, sheep and ox studied by banding techniques. Chromosoma 42: 383–402.
    Froenicke L, Caldes MG, Graphodatsky A et al. (2006) Are molecular cytogenetics and bioinformatics suggesting diverging models of ancestral mammalian genomes? Genome Research 16: 306–310.
    Glas R, Wakefield MJ, Toder R and Graves JA (1998) Comparative chromosome painting (Enclosed poster). ILAR Journal 39: INSERT.
    Goureau A, Yerle M, Schmitz A et al. (1996) Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36: 252–262.
    Hinsch H and Hannenhalli S (2006) Recurring genomic breaks in independent lineages support genomic fragility. BMC Evolutionary Biology 6: 90.
    Ijdo JW, Wells RA, Baldini A and Reeders ST (1991) Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Research 19: 4780.
    Jauch A, Wienberg J, Stanyon R et al. (1992) Reconstruction of genomic rearrangements in great apes and gibbons by chromosome painting. Proceedings of the National Academy of Sciences of the USA 89: 8611–8615.
    Lindblad-Toh K, Wade CM, Mikkelsen TS et al. (2005) Genome sequence, comparative analysis and haplotype structure of the domestic dog. Nature 438: 803–819.
    Mikkelsen TS, Wakefield MJ, Aken B et al. (2007) Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences. Nature 447: 167–177.
    Murphy WJ, Larkin DM, Everts-Van Der Wind A et al. (2005) Dynamics of mammalian chromosome evolution inferred from multispecies comparative maps. Science 309: 613–617.
    Pevzner P and Tesler G (2003) Genome rearrangements in mammalian evolution: lessons from human and mouse genomes. Genome Research 13: 37–45.
    Rettenberger G, Klett C, Zechner U et al. (1995) ZOO-FISH analysis: cat and human karyotypes closely resemble the putative ancestral mammalian karyotype. Chromosome Research 3: 479–486.
    Roberto R, Capozzi O, Wilson RK et al. (2007) Molecular refinement of gibbon genome rearrangements. Genome Research 17: 249–257.
    Ruiz-Herrera A, Garcia F, Mora L et al. (2005) Evolutionary conserved chromosomal segments in the human karyotype are bounded by unstable chromosome bands. Cytogenetic and Genome Research 108: 161–174.
    Ryder OA, Epel NC and Benirschke K (1978) Chromosome banding studies of the Equidae. Cytogenetics and Cell Genetics 20: 332–350.
    Scherthan H, Cremer T, Arnason U et al. (1994) Comparative chromosome painting discloses homologous segments in distantly related mammals. Nature Genetics 6: 342–347.
    Thomas R, Duke SE, Bloom SK et al. (2007) A cytogenetically characterized, genome-anchored 10-Mb BAC set and CGH array for the domestic dog. Journal of Heredity 98: 474–484.
    Wienberg J and Stanyon R (1997) Comparative painting of mammalian chromosomes. Current Opinion in Genetic Development 7: 784–791.
    Yang F, Carter NP, Shi L and Ferguson-Smith MA (1995) A comparative study of karyotypes of muntjacs by chromosome painting. Chromosoma 103: 642–652.
    Yang F, O_Brien PC, Milne BS et al. (1999) A complete comparative chromosome map for the dog, red fox, and human and its integration with canine genetic maps. Genomics 62: 189–202.
    Yue Y, Grossmann B, Ferguson-Smith M, Yang F and Haaf T (2005) Comparative cytogenetics of human chromosome 3q21.3 reveals a hot spot for ectopic recombination in hominoid evolution. Genomics 85: 36–47.
 Further Reading
    Griffin DK, Robertson LB, Tempest HG and Skinner BM (2007) The evolution of the avian genome as revealed by comparative molecular cytogenetics. Cytogenetic and Genome Research 117: 64–77.
    Gross M, Starke H, Trifonov V et al. (2006) A molecular cytogenetic study of chromosome evolution in chimpanzee. Cytogenetic and Genome Research 112: 67–75.
    Kearney L (2006) Multiplex-FISH (M-FISH): technique, developments and applications. Cytogenetic and Genome Research 114: 189–198.
    Kellogg ME, Burkett S, Dennis TR et al. (2007) Chromosome painting in the manatee supports Afrotheria and Paenungulata. BMC Evolutionary Biology 7: 6.
    Liehr T, Starke H, Heller A et al. (2006) Multicolor fluorescence in situ hybridization (FISH) applied to FISH-banding. Cytogenetic and Genome Research 114: 240–244.
    book O'Brien SJ, Menninger JC and Nash WG (eds) (2006) Atlas of Mammalian Chromosomes, 1st edn, Wiley-Liss. http://www.wiley.com/WileyCDA/Section/id-301698.html.
    Stanyon R, Bigoni F, Slaby T et al. (2004) Multi-directional chromosome painting maps homologies between species belonging to three genera of New World monkeys and humans. Chromosoma 113(6): 305–135.
    Svartman M, Stone G, Page JE and Stanyon R (2004) A chromosome painting test of the basal eutherian karyotype. Chromosome Research 12: 45–53.
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How to Cite close
Breen, Matthew, and Langford, Cordelia(Mar 2008) Comparative Cytogenetics. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0005801.pub2]