Cytogenetic and Physical Chromosomal Maps: Integration

Ilan R Kirsch, National Cancer Institute, Bethesda, Maryland, USA
Thomas Ried, National Cancer Institute, Bethesda, Maryland, USA

Published online: September 2006

DOI: 10.1002/9780470015902.a0005918

It is now possible to link a cytogenetic location directly with the sequence of the human genome. This is accomplished by using a toolset consisting of high-resolution fluorescent in situ hybridization-mapped and sequenced bacterial artificial chromosome clones distributed at 1–2 Mb intervals across the genome. This toolset is available from a commercial repository and is searchable in publicly available databases cross-referenced to extant information on cancer-associated chromosomal aberrations.

Keywords: cytogenetics; bacterial artificial chromosomes; fluorescent in situ hybridization; genome sequence; database

Figure 1. Localization of bacterial artificial chromosome (BAC) clones to a specific site on human prometaphase chromosomes. (Reproduced with permission from the National Cancer Institute, Cancer Genome Anatomy Project.)
Figure 2. Mapview display of BAC clone placement on chromosome 16.
Figure 3. FISH clone composite map of region 7pter–7p22.1.
Figure 4. An example of part of the Mitelman database.
Figure 5. The first page of the genomic sequence of bacterial artificial chromosome (BAC) clone RP11-297M9 which has been mapped using high-resolution fluorescent in situ hybridization (FISH) to 16p13.2a–16p13.2b. (Reproduced with permission from the National Center for Biotechnology Information and the National Cancer Institute, USA.)
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 Web Links
    ePath ResGen, Invitrogen Corporation http://www.resgen.com
    ePath Cancer Genome Anatomy Project, FISH Mapped BACs CCAP http://cgap.nci.nih.gov/Chromosomes/CCAP_BAC_Clones
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    ePath Developmental Gene Anatomy Project http://www.bwhpathology.org/dgap
    ePath OpenBiosystems http://www.openbiosystems.com
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Related Sub-Topics:

Bioinformatics | Molecular Genetics of Common and Complex Disease | Cancer Genetics | Techniques and Tools in Clinical Genetics | Chromosomal Disorders | Bioinformatics and Genetic Disease | Chromosomes and Chromatin Modifications | Techniques and Tools in Molecular Biology | DNA Damage and Repair | Computational Molecular Biology
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Article Title: Cytogenetic and Physical Chromosomal Maps: Integration
 
How to Cite close
Kirsch, Ilan R, and Ried, Thomas(Sep 2006) Cytogenetic and Physical Chromosomal Maps: Integration. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0005918]