Microarrays in Disease Diagnosis and Prognosis


Progress in microarray technologies will soon lead to their application in clinical practice. These high‐throughput technologies are expected to have a major impact on molecular pathology, initially in tumour classification and tumour staging.

Keywords: microarrays; CLL; TMA; SNP

Figure 1.

Surveillance of disease progression and response to therapy using gene expression profiling. Expression profiles were conducted with peripheral blood lymphocyte samples taken from 13 patients at different times. (a) Analysis of filtered expression data for nine patients (arbitrary labels) using singular value decomposition revealed a generic shift in messenger ribonucleic acid expression associated with disease progression, thus defining a progression axis spanned by the first two singular vectors PCA 1 and PCA 2 of the principle component analysis. In order to follow the development of disease in a single patient (‘follow‐up patient’), all samples taken from this patient were projected on to the same plane spanned by vectors PCA 1 and PCA 2. Temporally consecutive samples are connected with arrow‐marked lines. Thick lines denote the time frame of therapy. (b) Patient (arbitrary labels) with an initial 13q deletion acquires an additional 11q deletion resulting in progression of the disease. Therapy is able to improve the patient's status. (c) A patient (arbitrary labels) with initially constant disease status receives therapy resulting in improvement. However, during that time he acquires a 17p deletion, which results in progression of therapy‐resistant disease.



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Further Reading

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Alizadeh AA and Staudt LM (2000) Genomic‐scale gene expression profiling of normal and malignant immune cells. Current Opinion in Immunology 12: 219–225.

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Web Links

v‐erb‐b2 erythroblastic leukaemia viral oncogene homologue 2, neuro/glioblastoma derived oncogene homologue (avian) (ERBB2); Entrez ID: 2064. Link: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=full_report&list_udis=2064

Tumour protein p53 (Li–Fraumeni syndrome) (TP53); Entrez ID: 7157. Link: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=full_report&list_udis=7157

v‐erb‐b2 erythroblastic leukaemia viral oncogene homologue 2, neuro/glioblastoma derived oncogene homologue (avian) (ERBB2); MIM number: 164870. OMIM: http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=164870

Tumour protein p53 (Li–Fraumeni syndrome) (TP53); MIM number: 191170. OMIM: http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191170

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Stratowa, Christian(Jul 2007) Microarrays in Disease Diagnosis and Prognosis. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0005946.pub2]