Figure 1. Workflow of microarray design.

Figure 2. A screen capture of the visual display tool ‘Mondrian’ showing gene expression in the genomic region that contains the myosin-like gene. The top of the screen shows the combinations of data from which the user can select to customize the view. The genomic chromosome sequence is shown running from left to right. The amplicon squares indicate the position of each amplicon on the genomic sequence. Beneath the amplicon is indicated the gene expression of that sequence in each of the tissues and cell lines listed. The intensity of shading indicates the expression of the gene relative to that of the control, which in this example is a pool of tissue types. In each square is a white circle, with the size of this circle representing the relative intensity of the signal. Beneath the gene expression data, information from the gene prediction programs (Grail, GeneFinder and Genscan) is represented, along with data on the full-length clones generated by the in-house effort. Note that additional tissues were examined with microarrays (and are thus shown on the ‘Mondrian’), after the initial confirmatory data were collected in Figures 3

Figure 3. Expression data for the myosin-like gene: (a) microarray; (b) RT-PCR using RapidScan^TM Gene Expression tissues from Origene Technologies Inc. and (c) Northern blot analysis.

Figure 4. Gene structure of the myosin-like gene from human heart.