Cre–lox‐Inducible Gene Targeting

Abstract

Cre is a site‐specific DNA recombinase that is useful for either targeted excision or activation of a desired gene in the genome in a temporally and spatially controlled manner. Cre‐based recombination strategies also permit the design of precise chromosome translocations, deletions and duplications, which facilitate the genetic manipulation of the eukaryotic genome.

Keywords: transgenic mice; chromosome engineering; DNA recombinase; conditional mutation; embryonic stem cells

Figure 1.

loxP recombination site. The horizontal arrows indicate the two 13‐bp inverted repeats to which Cre binds.

Figure 2.

Conditional gene knockout. Black arrowheads represent the loxP sites, gray boxes represent the several exons of the target gene to be ablated and the open box represents the selectable marker neo. Also shown is a translational start in exon II (ATG) and a polyadenylylation site, (A)n, in exon III. For simplicity, excision of the neo gene is not shown: an option is removal of neo in ES cells after transient transfection with a cre plasmid by recombination at the two flanking loxP sites.

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References

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Further Reading

Austin S, Ziese M and Sternberg N (1981) A novel role for site‐specific recombination in maintenance of bacterial replicons. Cell 25: 729–736.

Craig NL, Craige R, Gellert M and Lambowitz AM (eds.) (2002) Mobile DNA II. Washington DC: American Society for Microbiology.

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How to Cite close
Sauer, Brian(Jan 2006) Cre–lox‐Inducible Gene Targeting. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005979]