Gene Targeting by Homologous Recombination

John H Wilson, Baylor College of Medicine, Houston, Texas, USA
Karen M Vasquez, University of Texas, M. D. Anderson Cancer Center, Science Park – Research Division, Smithville, Texas, USA

Published online: January 2006

DOI: 10.1038/npg.els.0005988

Homologous recombination involves the exchange of DNA molecules in regions of homology by a crossover event. Homologous recombination provides a mechanism for targeting defined modifications into genes of interest.

Keywords: homologous recombination; gene targeting; mouse disease models; genome modification; triplex technology

 References
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    Vasquez KM, Marburger K, Intody Z and Wilson JH (2001) Manipulating the mammalian genome by homologous recombination. Proceedings of the National Academy of Sciences of the United States of America 98: 8403–8410.
 Further Reading
    Anderson JK (2001) Genetically engineered mice and their use in aging research. Molecular Biotechnology 19: 45–47.
    Jonkers J and Berns A (2002) Conditional mouse models of sporadic cancer. Nature Reviews 2: 251–265.
    Lanzov VA (1999) Gene targeting for gene therapy: prospects. Molecular Genetics and Metabolism 68: 276–282.
    Paques F and Haber JE (1999) Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae. Microbiology and Molecular Biology Reviews 63: 349–404.
    Salvi M (2001) Shaping individuality: human inheritable germ line gene modification. Theoretical Medical Bioethics 22: 527–542.
    van de Steeg H, Vries A, van Oostrom Cth, et al. (2001) DNA repair-deficient Xpa and Xpa/p53+/– knock-out mice: nature of the models. Toxicology and Pathology 29(supplement): 109–116.
    Vasquez KM and Glazer PM (2002) Triplex-forming oligonucleotides: principles and applications. Quarterly Reviews of Biophysics 35: 89–107.
    Yanez RJ and Porter AC (1998) Therapeutic gene targeting. Gene Therapy 5: 149–159.
    Yu Y and Bradley A (2001) Engineering chromosomal rearrangements in mice. Nature Reviews Genetics 10: 780–790.

Related Sub-Topics:

Nucleic Acid Structure | Replication and Recombination | Gene and Genome Structure and Organisation | Techniques and Tools in Clinical Genetics | Genetic Models | DNA Structure and Function | Techniques and Tools in Molecular Biology | DNA Damage and Repair
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Article Title: Gene Targeting by Homologous Recombination
 
How to Cite close
Wilson, John H, and Vasquez, Karen M(Jan 2006) Gene Targeting by Homologous Recombination. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0005988]