Timothy J Griffin, Institute for Systems Biology, Seattle, Washington, USA
Jamie Sherman, University of Washington, Seattle, Washington, USA
Ruedi Aebersold, Institute for Systems Biology, Seattle, Washington, USA
Published online: January 2006
DOI: 10.1038/npg.els.0006196
An essential aspect of proteomic analysis is the identification and quantification of each protein present in two or more complex mixtures. The use of isotope-coded affinity tag (ICAT™) reagents and mass spectrometry forms part of quantitative proteomic analysis technology.
Keywords: quantitative proteomics; mass spectrometry; liquid chromatography; two-dimensional gel electrophoresis
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Figure 1. (a) Structure of the ICAT™ reagent; (b) quantitative analysis of protein mixtures using the ICAT™ reagent.
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| References | |
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| Further Reading | |
| Aebersold R and Goodlett DR (2001) Mass spectrometry in proteomics. Chemistry Review 101: 269–295. | |
| Gygi SP, Rist B, Griffin TJ, Eng J and Aebersold R (2002) Proteome analysis of low abundance proteins using multidimensional chromatography and isotope coded affinity tags. Journal of Proteome Research 1: 47–54. | |
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| Pandey A and Mann M (2000) Proteomics to study genes and genomes. Nature 405: 837–846. | |
| book Shabanowitz J, Settlage RE, Marto JA, et al. (2000) "Sequencing the primordial soup". In: Burlingame AL, Carr SA and Baldwin MA (eds.) Mass Spectrometry in Biology and Medicine, pp. 163–177. Totowa, NJ: Humana Press. | |
| Washburn MP, Wolters D and Yates III JR (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nature Biotechnology 19: 242–247. | |
| Yates III JR (2000) Mass spectrometry: from genomics to proteomics. Trends in Genetics 16: 5–8. | |
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