Rosamonde E Banks, Cancer Research UK, Leeds, UK
Rachel A Craven, Cancer Research UK, Leeds, UK
Peter J Selby, Cancer Research UK, Leeds, UK
Published online: September 2006
DOI: 10.1002/9780470015902.a0006206
Laser capture microdissection or laser-assisted microdissection techniques allow the purification or enrichment of selected cell types from heterogeneous tissue sections prior to downstream analysis of DNA, RNA or protein.
Keywords: proteomics; laser capture microdissection; laser-assisted microdissection; two-dimensional polyacrylamide gel electrophoresis; Western blotting
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Figure 1. Example of laser capture microdissection of a proximal tubule from a hematoxylin-and-eosin stained section of normal kidney showing (a) the intact section of renal cortex, (b) an enlarged area of proximal tubules, (c) the same area following microdissection of a proximal tubule and (d) the microdissected proximal tubule on the transfer cap.
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| References | |
| Banks RE, Dunn MJ, Forbes MA, et al. (1999) The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis: preliminary findings. Electrophoresis 20: 689–700. | |
| Craven RA, Totty N, Harnden P, Selby PJ and Banks RE (2002) Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis: evaluation of tissue preparation and sample limitations. American Journal of Pathology 160: 815–822. | |
| Emmert-Buck MR, Bonner RF, Smith PD, et al. (1996) Laser capture microdissection. Science 274: 998–1001. | |
| Emmert-Buck MR, Gillespie JW, Paweletz CP, et al. (2000) An approach to proteomic analysis of human tumors. Molecular Carcinogenesis 27: 158–165. | |
| Kolble K (2000) The LEICA microdissection system: design and applications. Journal of Molecular Medicine 78: B24–B25. | |
| Paweletz CP, Charboneau L, Bichsel VE, et al. (2001) Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene 20: 1981–1989. | |
| Paweletz CP, Ornstein DK, Roth MJ, et al. (2000) Loss of annexin 1 correlates with early onset of tumorigenesis in esophageal and prostate carcinoma. Cancer Research 60: 6293–6297. | |
| Schutze K and Lahr G (1998) Identification of expressed genes by laser-mediated manipulation of single cells. Nature Biotechnology 16: 737–742. | |
| Simone NL, Remaley AT, Charboneau L, et al. (2000) Sensitive immunoassay of tissue cell proteins procured by laser capture microdissection. American Journal of Pathology 156: 445–452. | |
| Wright Jr GL, Cazares LH, Leung S-M, et al. (1999) Proteinchip surface enhanced laser desorption/ionization (SELDI) mass spectrometry: a novel protein biochip technology for detection of prostate cancer biomarkers in complex protein mixtures. Prostate Cancer and Prostatic Diseases 2: 264–276. | |
| Further Reading | |
| Ahram M, Best CJ, Flaig MJ, et al. (2002) Proteomic analysis of human prostate cancer. Molecular Carcinogenesis 33: 9–15. | |
| Bohm M, Wieland I, Schutze K and Rubben H (1997) Microbeam MOMeNT: non-contact laser microdissection of membrane-mounted native tissue. American Journal of Pathology 151: 63–67. | |
| Eggeling F von, Davies H, Lomas L, et al. (2000) Tissue-specific microdissection coupled with ProteinChip array technologies: applications in cancer research. Biotechniques 29: 1066–1070. | |
| Fend F and Raffeld M (2000) Laser capture microdissection in pathology. Journal of Clinical Pathology 53: 666–672. | |
| Jones MB, Krutzsch H, Shu H, et al. (2002) Proteomic analysis and identification of new biomarkers and therapeutic targets for invasive ovarian cancer. Proteomics 2: 76–84. | |
| Lawrie LC, Curran S, McLeod HL, Fothergill JE and Murray GI (2001) Application of laser capture microdissection and proteomics in colon cancer. Molecular Pathology 54: 253–258. | |
| Maitra A, Wistuba II and Gazdar AF (2001) Microdissection and the study of cancer pathways. Current Molecular Medicine 1: 153–162. | |
| Natkunam Y, Rouse RV, Zhu S, Fisher C and van De RM (2000) Immunoblot analysis of CD34 expression in histologically diverse neoplasms. American Journal of Pathology 156: 21–27. | |
| Ornstein DK, Englert C, Gillespie JW, et al. (2000) Characterization of intracellular prostate-specific antigen from laser capture microdissected benign and malignant prostatic epithelium. Clinical Cancer Research 6: 353–356. | |
| Palmer-Toy DE, Sarracino DA, Sgroi D, LeVangie R and Leopold PE (2000) Direct acquisition of matrix-assisted laser desorption/ionization time-of-flight mass spectra from laser capture microdissected tissues. Clinical Chemistry 46: 1513–1516. | |
| Paweletz CP, Gillespie JW, Ornstein DK, et al. (2000) Rapid protein display profiling of cancer progression directly from human tissue using a protein biochip. Drug Development Research 49: 34–42. | |
| Web Links | |
| ePath University of Chicago (a useful website containing links to the main relevant sites) http://lcm.bsd.uchicago.edu/links.htm | |
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