Phosphoproteomics

Timothy D Veenstra, National Cancer Institute, Frederick, Maryland, USA
Thomas P Conrads, National Cancer Institute, Frederick, Maryland, USA

Published online: September 2006

DOI: 10.1002/9780470015902.a0006210

While proteomics aims to globally identify and measure the abundances of proteins, the challenge of phosphoproteomics is to determine globally the phosphorylation status of proteins present in the cell. Advances in mass spectrometry and developments in phosphopeptide enrichment methodologies is enabling high-throughput global identification and relative quantitation of phosphopeptides.

Keywords: phosphopeptide; phosphoprotein; phosphoproteome; mass spectrometry

Figure 1. Matrix assisted laser desorption/ionization-time of flight (MALDI TOF-TOF) tandem MS spectrum of a phosphopeptide. The molecular ion at m/z 2061.78 corresponds to the phosphopeptide FQpSEEQQQTEDELQDK from a -casein digest. The significant neutral loss of phosphate in the form of H3PO4 as a result of collision induced dissociation (CID) is quite apparent. It is still quite to observe almost the entire y series of ions produced in the CID process and, hence, the identity of the peptide is easily determined.
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 Further Reading
    Goshe MB, Conrads TP, Panisko EA, et al. (2001) Phosphoprotein isotope-coded affinity tags: application to the enrichment and identification of low-abundance phosphoproteins. Analytical Chemistry 74: 607–616.
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Related Sub-Topics:

Basic Cell Biology, Protein, RNA and DNA | Techniques in Cell Biology | Protein Structure | Mass Spectrometry | Biochemical and Biophysical Techniques | Proteins: Structure, Function, Metabolism
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Article Title: Phosphoproteomics
 
How to Cite close
Veenstra, Timothy D, and Conrads, Thomas P(Sep 2006) Phosphoproteomics. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0006210]