Genetic Mapping: Comparison of Direct and Indirect Approaches

Abstract

Two general approaches are available for investigating patterns of meiotic recombination in human germ cells: firstly, direct cytogenetic assessment of recombination in the cells in which it occurs; secondly, indirect genetic methods that exploit DNA polymorphisms. Each of these approaches has advantages and disadvantages for the production of genetic recombination maps.

Keywords: meiotic recombination; crossover; MLH1 foci; chiasmata; linkage analysis; map function; interference

References

Barlow AL and Hultén MA (1998) Crossing over analysis at pachytene in man. European Journal of Human Genetics 6: 350–358.

Broman KW, Murray JC, Sheffield VC, White RL and Weber JL (1998) Comprehensive human genetic maps: individual and sex‐specific variation in recombination. American Journal of Human Genetics 63: 861–869.

Cullen M, Perfetto SP, Klitz W, Nelson G and Carrington M (2002) High‐resolution patterns of meiotic recombination across the human major histocompatability locus. American Journal of Human Genetics 71: 759–776.

Hultén M (1974) Chiasma distribution at diakinesis in the normal human male. Hereditas 76: 55–78.

Hultén MA (1994) Chiasma formation, crossing‐over and recombination in meiosis. Trends in Genetics 10: 112–113.

Kong A, Gudbjartsson DF, Sainz J, et al. (2002) A high resolution recombination map of the human genome. Nature Genetics 31: 241–247.

Laurie DA and Hultén M (1985) Further chiasma studies on bivalent chiasma frequency in human males with normal karyotypes. Annals of Human Genetics 49: 189–201.

Lynn A, Koehler KE, Judis L, et al. (2002) Covariation of synaptonemal complex length and mammalian meiotic exchange rates. Science 296: 2222–2225.

Tease C, Hartshorne GM and Hultén MA (2002) Patterns of meiotic recombination in human fetal oocytes. American Journal of Human Genetics 70: 1469–1479.

Wallace BMN and Hultén MA (1985) Meiotic chromosome pairing in the normal human female. Annals of Human Genetics 49: 215–226.

Further Reading

Bishop D (2001) Meiotic recombination pathways. Encyclopedia of Life Sciences, http://www.els.net, London: Nature Publishing Group.

DeWan AT, Parrado AR, Marise TC and Leal SM (2002) The map problem: a comparison of genetic and sequence‐based physical maps. American Journal of Human Genetics 70: 101–107.

Hassold T, Sherman S and Hunt P (2000) Counting cross‐overs: characterizing meiotic recombination in mammals. Human Molecular Genetics 9: 2409–2419.

Hultén MA and Lawrie NM (1993) Chiasma density and interference maps of the normal and translocated chromosome 9 in the human male. First International Workshop on Chromosome 9. Annals of Human Genetics 56: 194–195.

Hultén MA, Palmer RW and Laurie DA (1982) Chiasma derived genetic maps and recombination fractions: chromosome 1. Annals of Human Genetics 46: 167–175.

Jorde LB (2000) Linkage disequilibrium and the search for complex diseases. Genome Research 10: 1435–1444.

Laurie DA, Palmer RW and Hultén MA (1982) Chiasma derived genetic lengths and recombination fractions: chromosomes 2 and 9. Annals of Human Genetics 46: 233–244.

Lichten M and Goldman ASH (1995) Meiotic recombination hotspots. Annual Review of Genetics 29: 423–444.

Petes TH (2001) Meiotic recombination hot spots and cold spots. Nature Reviews Genetics 2: 369.

Saadallah N and Hultén MA (1983) Chiasma distribution, genetic lengths and recombination fractions: a comparison between chromosome 15 and 16. Journal of Medical Genetics 20: 362.

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How to Cite close
Hultén, Maj, and Tease, Charles(Sep 2006) Genetic Mapping: Comparison of Direct and Indirect Approaches. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0006268]