Protein Production in Mammalian Cells

Abstract

Mammalian cells are an important host for the production of clinically relevant recombinant proteins. The most widely used approach for this purpose is to establish a cell line with an actively expressed recombinant gene stably integrated in its genome. Alternatively, protein can be transiently produced in cells for a few days immediately after deoxyribonucleic acid (DNA) transfer without the necessity of recombinant gene integration.

Keywords: recombinant protein; mammalian cells; DNA transfection; bioreactor; gene expression

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Further Reading

Adams GP and Weiner LM (2005) Monoclonal antibody therapy of cancer. Nature Biotechnology 23: 1147–1157.

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Dinnis DM and James DC (2005) Engineering mammalian cells factories for improved recombinant monoclonal antibody production: lessons from nature? Biotechnology and Bioengineering 91: 180–189.

Kwaks THJ and Otte A (2006) Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells. Trends in Biotechnology 24: 137–142.

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Wurm F and Bernard A (1999) Large‐scale transient expression in mammalian cells for recombinant protein production. Current Opinion in Biotechnology 10: 156–159.

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How to Cite close
Hacker, David L, and Wurn, Florian M(Sep 2007) Protein Production in Mammalian Cells. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0020209]