Protein Production in Mammalian Cells

David L Hacker, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Florian M Wurn, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

Published online: September 2007

DOI: 10.1002/9780470015902.a0020209

Mammalian cells are an important host for the production of clinically relevant recombinant proteins. The most widely used approach for this purpose is to establish a cell line with an actively expressed recombinant gene stably integrated in its genome. Alternatively, protein can be transiently produced in cells for a few days immediately after deoxyribonucleic acid (DNA) transfer without the necessity of recombinant gene integration.

Keywords: recombinant protein; mammalian cells; DNA transfection; bioreactor; gene expression

 References
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 Further Reading
    Adams GP and Weiner LM (2005) Monoclonal antibody therapy of cancer. Nature Biotechnology 23: 1147–1157.
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    Dinnis DM and James DC (2005) Engineering mammalian cells factories for improved recombinant monoclonal antibody production: lessons from nature? Biotechnology and Bioengineering 91: 180–189.
    Kwaks THJ and Otte A (2006) Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells. Trends in Biotechnology 24: 137–142.
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    book Smales CM and James DC (eds) (2005) Therapeutic Proteins: Methods and Protocols. Totowa, NJ: Humana Press.
    Wurm F and Bernard A (1999) Large-scale transient expression in mammalian cells for recombinant protein production. Current Opinion in Biotechnology 10: 156–159.

Related Sub-Topics:

Basic Cell Biology, Protein, RNA and DNA | Growth and Synthesis | Techniques in Cell Biology | Translation and Protein synthesis | Replication and Recombination | Techniques and Tools in Molecular Biology
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Article Title: Protein Production in Mammalian Cells
 
How to Cite close
Hacker, David L, and Wurn, Florian M(Sep 2007) Protein Production in Mammalian Cells. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0020209]