Protein Production in Mammalian Cells


Mammalian cells are an important host for the production of clinically relevant recombinant proteins. The most widely used approach for this purpose is to establish a cell line with an actively expressed recombinant gene stably integrated in its genome. Alternatively, protein can be transiently produced in cells for a few days immediately after deoxyribonucleic acid (DNA) transfer without the necessity of recombinant gene integration.

Keywords: recombinant protein; mammalian cells; DNA transfection; bioreactor; gene expression


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Further Reading

Adams GP and Weiner LM (2005) Monoclonal antibody therapy of cancer. Nature Biotechnology 23: 1147–1157.

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Hacker, David L, and Wurn, Florian M(Sep 2007) Protein Production in Mammalian Cells. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1002/9780470015902.a0020209]