Adaptive Evolution of Primate Sperm Proteins


Adaptive pressures throughout primate evolution have shaped sperm and seminal fluid proteins, causing them to change more rapidly than most other proteins. The adaptations are thought to be in response to sperm competition, sexual conflict, pathogen evasion and reinforcement.

Keywords: positive selection; adaptive evolution; sperm; semen; primate; sexual selection

Figure 1.

Steps of fertilization and specific proteins showing adaptive evolution.



Bezold G, Politch JA, Kiviat NB et al. (2007) Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia. Fertility and Sterility 87: 1087–1097.

Birtle Z, Goodstadt L and Ponting C (2005) Duplication and positive selection among hominin‐specific PRAME genes. BMC Genomics 6: 120.

Castle PE (2002) Could multiple low‐affinity bonds mediate primary sperm‐zona pellucida binding? Reproduction (Cambridge, England) 124: 29–32.

Civetta A (2003) Positive selection within sperm–egg adhesion domains of fertilin: an ADAM gene with a potential role in fertilization. Molecular Biology and Evolution 20: 21–29.

Clark NL, Aagaard JE and Swanson WJ (2006) Evolution of reproductive proteins from animals and plants. Reproduction 131: 11–22.

Clark NL and Swanson WJ (2005) Pervasive adaptive evolution in primate seminal proteins. PLoS Genetics 1: e35.

Dixson AL and Anderson MJ (2002) Sexual selection, seminal coagulation and copulatory plug formation in primates. Folia Primatol (Basel) 73: 63–69.

Dorus S, Evans PD, Wyckoff GJ, Choi SS and Lahn BT (2004) Rate of molecular evolution of the seminal protein gene SEMG2 correlates with levels of female promiscuity. Nature Genetics 36: 1326–1329.

Ford MJ (2001) Molecular evolution of transferrin: evidence for positive selection in salmonids. Molecular Biology and Evolution 18: 639–647.

Gasper J and Swanson WJ (2006) Molecular population genetics of the gene encoding the human fertilization protein zonadhesin reveals rapid adaptive evolution. American Journal of Human Genetics 79: 820–830.

Gibbs RA, Rogers J, Katze MG et al. (2007) Evolutionary and biomedical ibsights from the rhesus macaque genome. Science 316: 222–234.

Glassey B and Civetta A (2004) Positive selection at reproductive ADAM genes with potential intercellular binding activity. Molecular Biology and Evolution 21: 851–859.

Hamm D, Mautz BS, Wolfner MF, Aquadro CF and Swanson WJ (2007) Evidence of amino acid diversity‐enhancing selection within humans and among primates at the candidate sperm‐receptor gene PKDREJ. American Journal of Human Genetics 81: 44–52.

Herlyn H and Zischler H (2007) Sequence evolution of the sperm ligand zonadhesin correlates negatively with body weight dimorphism in primates. Evolution; International Journal of Organic Evolution 61: 289–298.

Jensen‐Seaman MI and Li WH (2003) Evolution of the hominoid semenogelin genes, the major proteins of ejaculated semen. Journal of Molecular Evolution 57: 261–270.

Kelly RW and Critchley HO (1997) Immunomodulation by human seminal plasma: a benefit for spermatozoon and pathogen? Human Reproduction 12: 2200–2207.

Kingan SB, Tatar M and Rand DM (2003) Reduced polymorphism in the chimpanzee semen coagulating protein, semenogelin I. Journal of Molecular Evolution 57: 159–169.

Kouprina N, Mullokandov M, Rogozin IB et al. (2004) The SPANX gene family of cancer/testis‐specific antigens: rapid evolution and amplification in African great apes and hominids. Proceedings of the National Academy of Sciences of the USA 101: 3077–3082.

Lundwall A and Olsson AY (2001) Semenogelin II gene is replaced by a truncated line 1 repeat in the cotton‐top tamarin. Biology of Reproduction 65: 420–425.

Miki K, Qu W, Goulding EH et al. (2004) Glyceraldehyde 3‐phosphate dehydrogenase‐S, a sperm‐specific glycolytic enzyme, is required for sperm motility and male fertility. Proceedings of the National Academy of Sciences of the USA 101: 16501–16506.

Nielsen R, Bustamante C, Clark AG et al. (2005) A scan for positively selected genes in the genomes of humans and chimpanzees. PLoS Biology 3: e170.

Olsson AY, Valtonen‐Andre C, Lilja H and Lundwall A (2004) The evolution of the glandular kallikrein locus: identification of orthologs and pseudogenes in the cotton‐top tamarin. Gene 343: 347–355.

Palumbi SR (1999) All males are not created equal: fertility differences depend on gamete recognition polymorphisms in sea urchins. Proceedings of the National Academy of Sciences 96: 12632–12637.

Podlaha O, Webb DM and Zhang J (2006) Accelerated evolution and loss of a domain of the sperm‐egg‐binding protein SED1 in ancestral primates. Molecular Biology and Evolution 23: 1828–1831.

Podlaha O and Zhang J (2003) Positive selection on protein‐length in the evolution of a primate sperm ion channel. Proceedings of the National Academy of Sciences of the USA 100: 12241–12246.

Qi H, Moran MM, Navarro B et al. (2007) All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility. Proceedings of the National Academy of Sciences of the USA 104: 1219–1223.

Ralt D, Goldenberg M, Fetterolf P et al. (1991) Sperm attraction to a follicular factor(s) correlates with human egg fertilizability. Proceedings of the National Academy of Sciences of the USA 88: 2840–2844.

Rooney AP and Zhang J (1999) Rapid evolution of a primate sperm protein: relaxation of functional constraint or positive Darwinian selection? Molecular Biology and Evolution 16: 706–710.

Rooney AP, Zhang J and Nei M (2000) An unusual form of purifying selection in a sperm protein. Molecular Biology and Evolution 17: 278–283.

Sabeti PC, Schaffner SF, Fry B et al. (2006) Positive natural selection in the human lineage. Science (New York) 312: 1614–1620.

Swanson WJ, Nielsen R and Yang Q (2003) Pervasive adaptive evolution in mammalian fertilization proteins. Molecular Biology and Evolution 20: 18–20.

Swanson WJ, Yang Z, Wolfner MF and Aquadro CF (2001) Positive Darwinian selection drives the evolution of several female reproductive proteins in mammals. Proceedings of the National Academy of Sciences of the USA 98: 2509–2514.

Torgerson DG, Kulathinal RJ and Singh RS (2002) Mammalian sperm proteins are rapidly evolving: evidence of positive selection in functionally diverse genes. Molecular Biology and Evolution 19: 1973–1980.

Vacquier VD (1998) Evolution of gamete recognition proteins. Science 281: 1995–1998.

Valtonen‐Andre C, Olsson AY, Nayudu PL and Lundwall A (2005) Ejaculates from the common marmoset (Callithrix jacchus) contain semenogelin and beta‐microseminoprotein but not prostate‐specific antigen. Molecular Reproduction and Development 71: 247–255.

Voight BF, Kudaravalli S, Wen XQ and Pritchard JK (2006) A map of recent positive selection in the human genome. PLoS Biology 4: 446–458.

Williamson SH, Hubisz MJ, Clark AG et al. (2007) Localizing recent adaptive evolution in the human genome. PLoS Genetics 3: e90.

Zelezetsky I, Pontillo A, Puzzi L et al. (2006) Evolution of the primate cathelicidin. Correlation between structural variations and antimicrobial activity. The Journal of Biological Chemistry 281: 19861–19871.

Further Reading

Balhorn R (2007) The protamine family of sperm nuclear proteins. Genome Biology 8: 227.

Birkhead TR (2000) Promiscuity : An Evolutionary History of Sperm Competition. Cambridge, MA: Harvard University Press.

Birkhead TR and Møller AP (1998) Sperm Competition and Sexual Selection. San Diego: Academic Press.

Eberhard WG (1996) Female Control: Sexual Selection by Cryptic Female Choice. Princeton, NJ: Princeton University Press.

Swanson WJ and Vacquier VD (2002) The rapid evolution of reproductive proteins. Nature Reviews Genetics 3: 137–144.

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How to Cite close
Clark, Nathaniel L(Apr 2008) Adaptive Evolution of Primate Sperm Proteins. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1002/9780470015902.a0020775]