Microscopy

To investigate the structure and function of the cell and its molecular components, it is necessary to integrate imaging techniques using optical, transmission, and scanning microscopy. This collection of articles describe how the development of microscopy and the implementation of new developments in microscopic imaging techniques have revolutionized biology.


Transmission Electron Microscopy: Preparation of Specimens
Bettina Böttcher
Published online: June 2012
Multiphoton Fluorescence Light Microscopy
Konstantinos Palikaras, Nektarios Tavernarakis
Published online: June 2012
Atomic Force Microscopy
Roland Galgoczy, Pere Roca‐Cusachs, Jordi Alcaraz
Published online: March 2013
Confocal Laser Scanning Light Microscopy
Stephen Paddock
Published online: April 2001
Confocal Microscopy
Ernst HK Stelzer
Published online: November 2001
Deconvolution Fluorescence Light Microscopy
Jason R Swedlow
Published online: March 2002
Differential Interference Contrast Light Microscopy
Ronald J Oldfield
Published online: April 2001
Electron Cryomicroscopy
Jacques Dubochet, Henning Stahlberg
Published online: April 2001
Electron Cryomicroscopy and Three‐dimensional Computer Reconstruction of Biological Molecules
Steven J Ludtke, Wah Chiu
Published online: June 2011
Electron Microscopy
John J Bozzola
Published online: July 2001
Far-field Light Microscopy
Christoph G Cremer
Published online: January 2006
Fluorescence Microscopy
Ken Jacobson
Published online: March 2010
Electronic and Computer Image Enhancement in Light Microscopy
Richard A Cardullo
Published online: April 2001
Fluorescence Microscopy
Hans J Tanke
Published online: January 2006
Immuno-electron Microscopy
Heinz Schwarz, Heinz Hohenberg
Published online: April 2001
Interference Reflection Microscopy
Igor Weber
Published online: January 2011
Light Microscopy
Ronald J Oldfield
Published online: April 2001
Light Microscopy – Brightfield and Darkfield Illumination
Ronald J Oldfield
Published online: January 2010
Light Microscopy Imaging Facilities
Jeremy Sanderson
Published online: January 2010
Mass Measurements by Scanning Transmission Electron Microscopy
David AD Parry
Published online: April 2001
Methods for Live Microscopy of Drosophila Spermatocytes
Matthew S Savoian, David M Glover
Published online: September 2008
Phase Contrast Microscopy
Jeremy B Sanderson
Published online: August 2001
Polarized Light Microscopy
Laura Finzi, David D Dunlap
Published online: April 2001
Single‐Molecule Light Microscopy
Karl Otto Greulich, Volker Uhl
Published online: March 2010
The Development of Fluorescence Microscopy
Barry R Masters
Published online: January 2010
Single Particle EM
José‐Jesús Fernández, José‐María Valpuesta
Published online: September 2009
Structure Determination by Single Particle Cryo‐electron Tomography
Thomas Hrabe, Friedrich Förster
Published online: February 2011
Immunofluorescence
Jean‐Marc Fritschy, Wolfgang Härtig
Published online: April 2001
Immunofluorescence: Protocol
JE Liddell
Published online: July 2003
Indirect Immunofluorescence of Cultured Cells
Jean‐Marc Fritschy, Wolfgang Härtig
Published online: June 2001
Indirect Immunofluorescence of Tissues
Wolfgang Härtig, Jean‐Marc Fritschy
Published online: April 2001
Immunofluorescence: Dyes and Other Haptens Conjugated with Antibodies
Wolfgang Härtig, Jean‐Marc Fritschy
Published online: September 2009