Techniques and Tools in Molecular Biology

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Pub Date A-Z
Somatic Cell Genetics
Frank Ruddle
Published online: May 2005
Electrophoresis and Blotting of DNA
Sandeep Tamber, Robert EW Hancock
Published online: March 2004
Homogenization of Soft Tissues (Rat Liver) in Iso‐osmotic Media
John M Graham
Published online: March 2004
Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation)
John M Graham, David Rickwood
Published online: March 2004
PCR-mediated Mutagenesis
J Chamberlain
Published online: March 2004
Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels
Edwin M Southern
Published online: July 2003
Direct Labelling of Proteins with 125I
Tor Gjøen, Trond Berg
Published online: July 2003
Separation of RNA and DNA in Methylmercuric Hydroxide Denaturing Agarose Gels
Edwin M Southern
Published online: July 2003
Alkaline Blotting of DNA Gels
Edwin M Southern
Published online: July 2003
Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane
J Beauchamp
Published online: July 2003
Manual Dot Blotting
J Beauchamp
Published online: July 2003
Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming
S Sheardown
Published online: July 2003
Mutation Rates: Data
Stewart T Schultz
Published online: July 2003
Capillary Blotting of RNA and DNA Gels
Edwin M Southern
Published online: July 2003
Restriction Mapping of Cloned DNA
K Woodward
Published online: July 2003
Nick Translation
K Woodward
Published online: July 2003
Transformation of Competent Bacterial Cells: Electroporation
R Anthony
Published online: July 2003
Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis
Edwin M Southern
Published online: July 2003
Electroblotting of Gels
Edwin M Southern
Published online: July 2003
Denaturation of RNA Using Glyoxal
Edwin M Southern
Published online: July 2003
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