Techniques and Tools in Molecular Biology

Pub Date A-Z
Alkaline Blotting of DNA Gels
Edwin M Southern
Published online: July 2003
Site‐directed Mutagenesis Using PCR
P Jones
Published online: July 2003
Restriction Enzyme Digests of DNA in Agarose Plugs for Pulsed Field Gel Electrophoresis
Edwin M Southern
Published online: July 2003
Isolation of DNA from Low Gelling Temperature Agarose
Edwin M Southern
Published online: July 2003
Denaturation of RNA Using Glyoxal
Edwin M Southern
Published online: July 2003
Radiolabelling Nucleic Acids: Generating DNA Probes by Random Priming
S Sheardown
Published online: July 2003
Restriction Mapping of Cloned DNA
K Woodward
Published online: July 2003
Transformation of Competent Bacterial Cells: Electroporation
R Anthony
Published online: July 2003
Manual Dot Blotting
J Beauchamp
Published online: July 2003
Mutation Rates: Data
Stewart T Schultz
Published online: July 2003
Capillary Blotting of RNA and DNA Gels
Edwin M Southern
Published online: July 2003
Separation of RNA and DNA in Methylmercuric Hydroxide Denaturing Agarose Gels
Edwin M Southern
Published online: July 2003
Electroblotting of Gels
Edwin M Southern
Published online: July 2003
Nick Translation
K Woodward
Published online: July 2003
Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane
J Beauchamp
Published online: July 2003
Isolation of Plasmid DNA from E. coli
P Jones
Published online: May 2003
Whole Animal Cloning
Ian Wilmut
Published online: May 2003
Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA
Edwin M Southern
Published online: May 2003
Recovery of DNA from Polyacrylamide or Agarose by Electroelution
P Jones
Published online: May 2003
Patenting Genes and their Products
Nick Buffinger, Desmond M Mascarenhas
Published online: May 2003
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