Techniques and Tools in Molecular Biology

Pub Date A-Z
Manifold Dot or Slot Blotting of DNA on to Nitrocellulose or Uncharged Nylon Membrane
J Beauchamp
Published online: July 2003
Capillary Blotting of RNA and DNA Gels
Edwin M Southern
Published online: July 2003
Restriction Mapping of Cloned DNA
K Woodward
Published online: July 2003
Transformation of Competent Bacterial Cells: Electroporation
R Anthony
Published online: July 2003
Quantification of DNA and RNA: A Spectrophotometric Method
Bernhard Lieb
Published online: July 2003
Site‐directed Mutagenesis Using PCR
P Jones
Published online: July 2003
Denaturation of RNA Using Glyoxal
Edwin M Southern
Published online: July 2003
Isolation of Plasmid DNA from E. coli
P Jones
Published online: May 2003
Patenting Genes and their Products
Nick Buffinger, Desmond M Mascarenhas
Published online: May 2003
Whole Animal Cloning
Ian Wilmut
Published online: May 2003
Electrophoresis of DNA and RNA in Non‐denaturing Vertical Polyacrylamide Gels
Edwin M Southern
Published online: May 2003
Denaturing Formamide Polyacrylamide Gels for Separating RNA and DNA
Edwin M Southern
Published online: May 2003
Recovery of DNA from Polyacrylamide or Agarose by Electroelution
P Jones
Published online: May 2003
Northern Blotting
L Woodgate, K Mills
Published online: March 2003
Isolation of DNA from Agarose Gels using Agarase
Edwin M Southern
Published online: March 2003
Translation of mRNAs in Xenopus Oocytes
Brett D Keiper
Published online: March 2003
Reverse Transcription and PCR
L Woodgate, K Mills
Published online: March 2003
Autoradiography and Fluorography
Pavel S Gromov, Julio E Celis
Published online: March 2003
Pulsed‐field Gel Electrophoresis of DNA
Edwin M Southern
Published online: March 2003
Southern Blotting for the Analysis of Human Disease
Rheem D Medh
Published online: March 2003
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