ATPases: Ion‐motive


Ion motive ATPases allow ion(s) to accumulate on one side of a biological membrane at the expense of ATP hydrolysis. In this way active transport across biological membranes is mediated.

Keywords: active transport; ion; ATP hydrolysis; pump structure; mechanism

Figure 1.

Ca2+ ATPase tertiary structure prediction, redrawn from Green and Stokes . Note the phosphorylation site (D, aspartate) and the nucleotide site (K, lysine)

Figure 2.

Ca2+ ATPase shape, redrawn from Toyoshima et al. .

Figure 3.

E1/E2 model for ion transport.

Figure 4.

Function of Na+/K+ ATPase.

Figure 5.

V‐ATPase motor, redrawn from Boekema et al. . ATP hydrolysis in V1 headpiece induces rotation of the V1 stalk which in turn induces rotation of the V0 rotor. This mechanical energy is used for H+ translocation.



Boekema EJ, Ubbink‐Kok T, Lolkema JS, Brisson A and Konings WN (1997) Visualisation of a peripheral stalk in V‐type ATPase: evidence for the stator structure essential to rotational catalysis. Proceedings of the National Academy of Sciences of the USA 94: 14291–14293.

Davis BA, Edes I, Gupta RC et al. (1990) The role of phospholamban in the regulation of calcium transport by cardiac sarcoplasmic reticulum. Molecular and Cellular Biochemistry 99: 83–88.

Finbow ME and Harrison MA (1997) The vacuolar H+‐ATPase: a universal proton pump of eukaryotes. Biochemical Journal 324: 697–712.

Green NM and Stokes DL (1992) Structural modelling of P‐type ion pumps. Acta Physiologica Scandinavia 146: 59–68.

Jencks WP (1989) How does a calcium pump pump calcium? Journal of Biological Chemistry 264: 18855–18858.

Lingrel JB and Kuntzweiler T (1994) Na+, K+‐ATPase. Journal of Biological Chemistry 269: 19659–19662.

Mintz E and Guillain (1997) Ca2+ transport by the sarcoplasmic reticulum ATPase. Biochimica et Biophysica Acta 1318: 52–97.

Nakamoto RK and Slayman CW (1989) Molecular properties of the fungal plasma‐membrane [H+]‐ATPase. Journal of Bioenergetics and Biomembranes 21: 621–632.

Toyoshima C, Sasabe H and Stokes DL (1993) Three‐dimensional cryo‐electron microscopy of the calcium ion pump in the sarcoplasmic reticulum membrane. Nature 362: 469–471.

Wallmark B, Lorentzon P and Sachs G (1990) The gastric H+, K+‐ATPase. Journal of Internal Medicine 228 (supplement 1): 3–8.

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Further Reading

Bittar EE and Andersen JP (eds) (1998) Ion pumps. A special volume of Advances in Molecular and Cell Biology, vols 23A and 23B. London: JAI Press.

Skou JC and Esmann M (1992) The Na+, K+‐ATPase. Journal of Bioenergetics and Biomembranes 24: 249–261.

Solioz M and Vulpe C (1996) CPx‐type ATPases: a class of P‐type ATPases that pump heavy metals. Trends in Biochemical Sciences 21: 237–241.

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How to Cite close
Guillain, Florent, and Mintz, Elisabeth(Apr 2001) ATPases: Ion‐motive. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0000678]