Cell Culture: Basic Procedures


Cell culture has provided a valuable research tool for over 100 years. Cell culture is a broad term applied to the wide range of techniques to culture cells on an artificial nutrient medium in a controlled culture environment.

Keywords: culture medium; primary cell culture; cell culture equipment; cryopreservation

Further Reading

Barnes D and Sirbasku D (eds) (1987) Peptide growth factors. Methods In Enzymology, vol. 146, part A; vol. 147, part B. New York: Academic Press.

Barnes D, Sirbasku D and Sato G (eds) (1984) Cell Culture Methods For Molecular and Cell Biology, 4 vols. New York: Wiley‐Liss.

Barnes D, Mather J and Sato G (eds) (1991) Peptide growth factors. Methods In Enzymology, vol. 198, part C. New York: Academic Press.

Butler MJ (1997) Animal Cell Culture and Technology. London: IRL Press.

Darling DC and Morgan SJ (1994) Animal Cells: Culture and Media. Chichester, UK: Wiley.

Darling DC and Morgan SJ (1994) Animal Cell Culture: Introduction to Biotechniques. Oxford: Bios.

Doyle D, Hay R and Kirsop BE (eds) (1991) Animal Cells: Living Resources for Biotechnology. Cambridge: Cambridge University Press.

Freshney RI (ed.) (1992) Animal Cell Culture: A Practical Approach. London: IRL Press.

Freshney RI (1994) Culture of Animal Cells: A Manual of Basic Techniques, 3rd edn. New York: Wiley‐Liss.

Harrison MA and Rae IF (1997) General Techniques of Cell Culture (Handbooks in Practical Animal Cell Biology). Cambridge: Cambridge University Press.

Jakoby WB and Pastan IH (1979) Cell culture. Methods in Enzymology, vol. 58. New York: Academic Press

Mather J and Barnes D (eds) (1998) Animal cell culture. Methods In Cell Biology, vol. 57. New York: Academic Press.

Mather JP and Roberts PE (1998) Introduction to Cell and Tissue Culture: Theory and Technique. New York: Plenum.

Murakami H, Yamane I, Hayashi I et al. (eds) (1985) Growth and Differentiation of Cells in Defined Environments. Berlin: Springer.

Pollard JW and Walker JM (1990) Animal Cell Culture. Methods in Molecular Biology, vol. 5. Totowa, NJ: Humana Press.

Various editors (1989–current) Proceedings of the Annual Meeting of the Japanese Association for Animal Cell Technology. Dordrecht: Kluwer.

Wasley JD and May JW (1971) Animal Cell Culture Methods. Philadelphia: Lippincott‐Raven.

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Mericko, Patricia, Helmirch, Angela, Chen, LuPeng, Kusamoto, Kenichi, Toumadje, Araz, Chapline, Christine, Sato, Denry, and Barnes, David W(May 2002) Cell Culture: Basic Procedures. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002556]