Quantitative Proteomics (ICAT™)


An essential aspect of proteomic analysis is the identification and quantification of each protein present in two or more complex mixtures. The use of isotope‐coded affinity tag (ICAT™) reagents and mass spectrometry forms part of quantitative proteomic analysis technology.

Keywords: quantitative proteomics; mass spectrometry; liquid chromatography; two‐dimensional gel electrophoresis

Figure 1.

(a) Structure of the ICAT™ reagent; (b) quantitative analysis of protein mixtures using the ICAT™ reagent.



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Further Reading

Aebersold R and Goodlett DR (2001) Mass spectrometry in proteomics. Chemistry Review 101: 269–295.

Gygi SP, Rist B, Griffin TJ, Eng J and Aebersold R (2002) Proteome analysis of low abundance proteins using multidimensional chromatography and isotope coded affinity tags. Journal of Proteome Research 1: 47–54.

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Washburn MP, Wolters D and Yates III JR (2001) Large‐scale analysis of the yeast proteome by multidimensional protein identification technology. Nature Biotechnology 19: 242–247.

Yates III JR (2000) Mass spectrometry: from genomics to proteomics. Trends in Genetics 16: 5–8.

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How to Cite close
Griffin, Timothy J, Sherman, Jamie, and Aebersold, Ruedi(Jan 2006) Quantitative Proteomics (ICAT™). In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0006196]