While proteomics aims to globally identify and measure the abundances of proteins, the challenge of phosphoproteomics is to determine globally the phosphorylation status of proteins present in the cell. Advances in mass spectrometry and developments in phosphopeptide enrichment methodologies is enabling high‐throughput global identification and relative quantitation of phosphopeptides.

Keywords: phosphopeptide; phosphoprotein; phosphoproteome; mass spectrometry

Figure 1.

(MALDI TOF‐TOF) tandem MS spectrum of a phosphopeptide. The molecular ion at m/z 2061.78 corresponds to the phosphopeptide FQpSEEQQQTEDELQDK from a β‐casein digest. The significant neutral loss of phosphate in the form of H3PO4 as a result of collision induced dissociation (CID) is quite apparent. It is still quite to observe almost the entire y series of ions produced in the CID process and, hence, the identity of the peptide is easily determined.



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Further Reading

Goshe MB, Conrads TP, Panisko EA, et al. (2001) Phosphoprotein isotope‐coded affinity tags: application to the enrichment and identification of low‐abundance phosphoproteins. Analytical Chemistry 74: 607–616.

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Veenstra, Timothy D, and Conrads, Thomas P(Sep 2006) Phosphoproteomics. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1002/9780470015902.a0006210]